| | 1 | Author
| Ahlert Schmidt | Requires cookie* | | | Title
| Occurrence of Mercaptopyruvate Sulfotransferase Activity in Photosynthetic Organisms  | | | | Abstract
| Mercaptopyruvate sulfotransferase activity catalyzes the formation o f pyruvate from mercapto pyruvate in the presence o f suitable reagents as acceptor. It was detected in Lemna minor, Pisum sativum, Spinacia oleracea, Chlorella fusca, Synechococcus 6301, and Rhodopseudomonas palustris. Best activity was detected using dithioerythritol as a thiol reagent; good activity was obtained using mercaptoethanol, glutathione, mercaptopyruvate or sulfite as acceptor. The pH-optimum for the Chlorella mercaptopyruvate sulfotransferase was found around 9; the apparent Km for mercaptopyruvate was determined to 2 m M and for dithioerythritol for 5 m M using crude Chlorella extracts. The role of this enzyme is discussed in relation to cysteine catabolism by photosynthetic organisms. | | | |
Reference
| Z. Naturforsch. 39c, 916—921 (1984); received April 6 1984 | | | |
Published
| 1984 | | | |
Keywords
| Pyruvate, Mercaptopyruvate, Lemna, Spinacia, Pisum, Chlorella, Synechococcus, Rhodopseudomonas | | | |
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| default:Reihe_C/39/ZNC-1984-39c-0916.pdf | | | | Identifier
| ZNC-1984-39c-0916 | | | | Volume
| 39 | |
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