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1980 (1)
1976 (1)
1Author    G. W. PohlRequires cookie*
 Title    Spectral Properties of Fluorescent Dyes in Lecithin Vesicles Probes for the Structure of Lipid Bilayer Membranes and for Membrane Potentials  
 Abstract    Three fluorescent dyes (a merocyanin, a cyanin and a modified umbelliferon) have been in­ corporated into vesicles from three different lecithins. Their absorption spectrum, emission spectrum and fluorescence polarization in the lipid vesicles has been studied and compared with the spectral properties in homogeneous organic solutions. The reactivity of lipid bound dyes with reagents in the aqueous phase was investigated. The location use as indicators of phase transitions or membrane 
  Reference    (Z. Naturforsch. 31c, 575 [1976]; received May 10 1976) 
  Published    1976 
  Keywords    Lecithin Vesicles, Fluorescent Probes, Phase Transitions, Membrane Potentials 
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 TEI-XML for    default:Reihe_C/31/ZNC-1976-31c-0575.pdf 
 Identifier    ZNC-1976-31c-0575 
 Volume    31 
2Author    W. G. Pohl, R. K. Reikenbohm, K. SeuwenRequires cookie*
 Title    The Specificity of Ionophore A 23187 in Cation Transport across Lipid Membranes Studies with Lecithin Vesicles  
 Abstract    Lecithin vesicles containing different cations were prepared by sonication and characterized. When the concentration o f the divalent cations in the buffer was increased from 1 m M to about 100 m M the cation-concentration inside o f the vesicles did not increase proportional to the outside concentration, but showed a saturation behaviour. The efflux o f various cations m ediated by ionophore A 23187 was m easured and relative transport-rates were determined. The following sequence was obtained: Zn2+ > Mn2+ > Ca2+ > M g2+ > Sr2+ > Ba2+ % L i+ > N a +. The efflux o f Ca2+ increased proportional to the square o f the A 2 3 187-concentration. To lecithin-vesicles containing ethylenediam ine tetraacetate (ED TA) inside different cations were added on the outside. In the presence o f A 23187 divalent cations are transported into the vesicles and bound there by EDTA. During the influx o f M e2+-ions into vesicles a H +-efflux was observed. The resulting pH-decrease was measured. The rate o f the pH -change depended on the M e2+-ion used. The sequence was: Cd2+ > Zn2+ > Ca2+ > M n2+ > M g2+ > Sr2+, Ba2+. 
  Reference    Z. Naturforsch. 35c, 562 (1980); received February 11/M arch 6 1980 
  Published    1980 
  Keywords    Ionophore A 23187, Lecithin Vesicles, Cation Specificity, Proton Antiport 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0562.pdf 
 Identifier    ZNC-1980-35c-0562 
 Volume    35