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1990 (1)
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1Author    GerardJ. Niem, WimJ. Baas, Elisabeth Besson, JeanC. HopinRequires cookie*
 Title    Investigations on Hoya Species. III. Leaf Phenolics and Latex Lipids of Hoya lacunosa Bl.  
 Abstract    Hoya lacunosa leaves contain some main C-glycosylflavonoids, which were identified as: 6-C-arabinosyl 8-C-glucosylapigenin (isoschaftoside), 6-C-glucosyl 8-C-arabinosylapigenin (schaftosi-de) and 6,8-di-C-arabinosylapigenin. In the latex triterpenols were found, often both free and in ester form, of which /?-amyrin, a-amyrin, lupeol, 24-methylenecycloartanol, obtusifoliol and cyclo-eucalenol were identified in the free alcohol fraction. The esters, forming the m ajor part o f the to­ tal lipid fraction, were solely acetates o f some of the above mentioned alcohols. 
  Reference    Z. Naturforsch. 34c, 1125—1128 (1979); received August 31 1979 
  Published    1979 
  Keywords    Hoya lacunosa, Asclepiadaceae, C-Glycosylflavonoids, Latex, Triterpenoids, 4-ar-Methylsterols 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-1125.pdf 
 Identifier    ZNC-1979-34c-1125 
 Volume    34 
2Author    B. C. Homeyer, MargaretF. RobertsRequires cookie*
 Title    Alkaloid Sequestration by Papaver somniferum Latex  
 Abstract    The 1000 x g organelles o f Papaver somniferum latex have been shown to accum ulate rapidly, large quantities o f alkaloids (0 .0 5 -1 m g/m g organelle protein), particularly m orphine, thebaine, codeine and papaverine and have been found to exhibit a surprising degree o f specificity for the accumulation o f these alkaloids. The uptake o f alkaloid is independent o f temperature and does not show a requirement for ATP 10_ 3 m, M g2+ 10" 3 M , or KC1 10_ 3 m. It is unaffected by known ATPase inhibitors (chlorm adinone acetate 2 .5 x 1 0 _ 5 m, N ,N -dicyclohexylcarbo-diim ide 5 x 10-4 M and sodium vanadate 10_ 4 m) but showed considerable increase in m orphine accumulation in the presence o f 4-chlorom ercum benzoate 5 x 10-4 M , w hile N -m ethylm aleim id e 5 x 10_ 4 m had no effect, and carbonylcyanide 4-(trifluorom ethoxy)phenylhydrazone 5 x 10_ 4 m caused a reduction (ca. 30%) o f m orphine uptake. An acid m edium , pH 4 .5 -5 .5 , also results in decreased morphine uptake. 
  Reference    Z. Naturforsch. 39c, 876 (1984); received M ay 21/June 18 1984 
  Published    1984 
  Keywords    Papaver somniferum L, Alkaloid, Sequestration, Alkaloid Uptake, Organelle, Latex 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0876.pdf 
 Identifier    ZNC-1984-39c-0876 
 Volume    39 
3Author    M. Arie-Theres, Hauser, Michael WinkRequires cookie*
 Title    Uptake of Alkaloids by Latex Vesicles and Isolated Mesophyll Vacuoles of Chelidonium ntajus (Papaveraceae)  
 Abstract    Latex vacuoles o f Chelidonium majus (Papaveraceae) naturally sequester protoberberine and benzophenanthridine alkaloids. Isolated vesicles take up a number o f other heterologous alkaloids, such as 9,10-dihydroergocryptine, vinblastine, strychnine, nicotine, colchicine, lupanine, and 13-hydroxylupanine, with a strong preference for the indole alkaloids and for nicotine. Kinetic data clearly show that the uptake proceeds via simple diffusion and not by carrier-mediated transport. The alkaloids are concentrated in the vesicles against a concentra­ tion gradient. M gA TP has no influence on alkaloid uptake. It is likely that alkaloids are trapped in the vesicles by chelidonic acid (concentration in latex 58 mM, in vesicles 661 mM) which com plexes these alkaloids and thus prevents their rediffusion out o f the vesicles. Uptake o f these alkaloids by leaf protoplasts and vacuoles o f Ch. majus, by vacuoles o f suspension-cultured cells o f Lupinuspolyphyllus and by rabbit erythrocytes was studied for comparison. 
  Reference    Z. Naturforsch. 45c, 949 (1990); received January 15/May 18 1990 
  Published    1990 
  Keywords    Chelidonium m ajus, Alkaloid Uptake, Vacuole, Latex, Diffusion, Ion-Trap 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-0949.pdf 
 Identifier    ZNC-1990-45c-0949 
 Volume    45