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1982 (1)
1Author    P. Moesta, U. Seydel, B. Lindner, H. G. RisebachRequires cookie*
 Title    Detection of Glyceollin on the Cellular Level in Infected Soybean by Laser Microprobe Mass Analysis  
 Abstract    Laser microprobe mass analysis was explored as a m ethod for the detection at the cellular level of the phytoalexin glyceollin in soybean cotyledons infected with an incom patible race of Phy­ tophthora megasperma f. sp. glycinea. For LAMMA® analysis 10 |im freeze microtome sections which were freeze-dried on copper grids were used. The LAM M A spectrum of glyceollin (isomer I) shows a characteristic peak at m /e = 321 which can be attributed to M -O H +. This peak was also present in the spectra of infected regions from a cotyledon b u t was completely absent in the spec­ tra of uninfected tissue. One hundred and fifty LAM M A spectra were taken along a line per­ pendicular to the border line o f infection. A steep rise in glyceollin content toward the infected area was observed. This is the First tim e th at such highly localized glyceollin accumulation has been shown at the cellular level. The results show th at LAM M A analysis is suitable for the de­ tection of organic molecules in biological tissues w ith high lateral resolution. 
  Reference    Z. Naturforsch. 37c, 748—751 (1982); received May 19 1982 
  Published    1982 
  Keywords    Glyceollin, Phytoalexins, Soybean, Glycine m ax, L, LAM MA 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0748.pdf 
 Identifier    ZNC-1982-37c-0748 
 Volume    37