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'Juvenile Hormone' in keywords Facet   section ZfN Section C  [X]
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1984 (2)
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1Author    K. H. Trautmann, A. Schuler, Dr Maag, Ag Dielsdorf, SchweizM. Suchý, H.-K WipfRequires cookie*
 Title    Eine  
 Abstract    Methode zur qualitativen und quantitativen Bestimmung von drei Juvenilhormonen von Insekten Nachweis von 10.1 l-Epoxy-3.7.1 l-trimethyl-2-frans-6-//-ons-dodecadiensäuremethyl-ester in Melolontha melolontha A Method for the Qualitative and Quantitative Determination of Three Natural Insect Juvenile Hormones Evidence of Methyl 10,ll-epoxy-3,7,ll-trimethyl-2-/rans-6-*ran.s-dodecadienoate in Melolontha melolontha A method is presented permitting the qualitative and quantitative determination of all three presently known hormones (J H 1 —3). The determination is based on the method of radioactive isotope dilution, whereby a very small known amount of tritium-labelled JH-1 is added to the ether extract of the particular species. The addition of radioactive JH-1 permits the isolation of all three hormones, because of their similar behaviour during the chosen work up. The quantitative determination was carried out by gas chromatography and the identification was confirmed with the help of retention-times and GC-MS combination. The method was checked by using an extract of Hyalophora cecropia. For the first time methyl 10,ll-epoxy-3,7,ll-trimethyl-2-frans-6-/rans-dodecadienoate (JH-3) could also be identified as the juvenile hormone of Melo­ lontha melolontha. In Vanessa io larvae, Tenebrio molitor larvae and adults and in Musca domestica larvae none of the three known hormones could be detected. The preparation of JH-1 labelled with tritium in the methyl group of the ester was accomplished with very high specific activity (4.34 Ci/mmol) of the tritiated acid with diazomethane. 
  Reference    (Z. Naturforsch. 29c, 161—168 [1974]; eingegangen am 12. Dezember 1973) 
  Published    1974 
  Keywords    Quantitative Determination, Insects, Juvenile Hormones 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0161.pdf 
 Identifier    ZNC-1974-29c-0161 
 Volume    29 
2Author    L. W. Van Broekhoven, A. C. Van Der Kerk, -Van Hoof, C. A. SaleminkRequires cookie*
 Title    Gas Chromatographie Determination of Subnanogram Amounts of the Juvenile Hormone Methyl (2E, 6E)-10,ll-epoxy-3,7,ll-tri- methyl-2,6-dodecadienoate (JH III) in Insect Material by Electron Capture Detection  
 Abstract    Using an electron capture detector, a sensitive gas chromatographic method has been developed for the determination of the juvenile hormone methyl(2E, 6E)-lOjll-epoxy-SJjll-trimethyl-^ö-dodecadienoate (JH III) in insect material. The sensitivity in electron capture detection of the bisheptafluorobutyrate was determined. The work-up procedure was checked by adding known amounts of JH III to insect material. The method seems equally applicable to all three juvenile hormones. 
  Reference    (Z. Naturforsch. 30c, 726—729 [1975]; received May 28 1975) 
  Published    1975 
  Keywords    Quantitative Determination, Electron Capture Detection, Insects, Juvenile Hormone 
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 TEI-XML for    default:Reihe_C/30/ZNC-1975-30c-0726.pdf 
 Identifier    ZNC-1975-30c-0726 
 Volume    30 
3Author    G. S. Dogra, G. M. Ulrich, H. RemboldRequires cookie*
 Title    A Comparative Study of the Endocrine System of the Honey Bee Larvae under Normal and Experimental Conditions  
 Abstract    The endocrine system of the honey bee (Apis mellifera L.) has been studied morphologically through post-embryonic development with several histological techniques. Marked differences in the structure of the neurosecretory complex of queen and worker larvae have been observed during larval stages. In queen larvae, morphogenesis of the neurosecretory cells, their axons and the formation of the chiasma takes place during end of 2nd and beginning of 3rd, in the workers at beginning of 4th larval instar. Stainable neurosecretory material was found in queen larvae at the beginning, in worker larvae at the end of 4th instar. In early larval stages, the corpora allata are more active in the queen. During initial 3 — 5 days of larval development the gland volume is reduced in both castes. After 36 to 48 hours of endocrine retardation, the glands become active again. The same histological effects are found under experimental conditions, where worker larvae of 2nd instar were reared in the incubator on basic food, Royal Jelly and with topically applied juvenile hormone I. 
  Reference    (Z. Naturforsch. 32c, 637 [1977]; received February 9 1976/April 4 1977) 
  Published    1977 
  Keywords    Insect, Endocrinology, Honey Bee, Caste Determination, Juvenile Hormone 
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 TEI-XML for    default:Reihe_C/32/ZNC-1977-32c-0637.pdf 
 Identifier    ZNC-1977-32c-0637 
 Volume    32 
4Author    Jean-Paul Delbecque, Karel SlámaRequires cookie*
 Title    Ecdysteroid Titres during Autonomous Metamorphosis in a Dermestid Beetle  
 Abstract    The larvae of Dermestes maculatus are able to undergo several moults in complete absence o f the cephalic and prothoracic neuroendocrine system, showing thus spontaneous metamorphosis. Measurements of ecdysteroid contents by radioimmunoassay methods have revealed distinctive larval, prepupal and pupal ecdysteroid peaks, as found in other species where development requires frequent stimulation by the centrally produced hormones. Mass fragmentographic analysis has indicated that more than 80% of the ecdysteroids detected by the radioimmunoassay during the peaks consists of ecdysterone. The prothoracic glands (PG) o f D. maculatus larvae are located in the head capsule and in the cervical region. They can be separated from the body by neck-ligation. Only the larval peak o f ecdysterone can be correlated with PG function. The prepupal and pupal peaks o f ecdysterone are not produced by the central neuroendocrine system. The isolated abdominal fragments o f larvae and pupae positively produce ecdysteroids in the course of their independent development, but this synthesis has been subnormal and occasionally delayed in time. In contrast, the isolated thoracic fragments produce well synchronized peaks o f ecdysterone and this does not depend on the PG. It thus appears that some thoracic tissue other than PG is able to maintain the physiological concentration o f ecdysterone by means o f a concentration-dependent feed-back mechanism. Due to spontaneous metamorphosis and extremely good survival we have succeeded to induce in this species: a) premature formation o f the prepupal cycle of ecdysterone synthesis in the penultimate larval instar by ligation; b) inhibition and delay o f the prepupal peak by means of juvenoid treatments; c) reappearance o f the prepupal peak in the inhibited "permanent larvae" by exogenous administration of ecdysterone, or; d) modification of the pupal peak by a qualitatively new pupal-pupal one after juvenoid treatments. These experimental transformations o f the ecdysterone peaks have suggested that the kind and nature o f the peaks are closely related to nature and stage o f the ontogenetic development. We therefore believe that the process o f larval-pupal reprogramming has occurred in this species long before the increase o f ecdysterone titer in the body. Both the prepupal and pupal peaks do not represent a cause but they show to be rather consequences o f the developmental programming. Further features associated with possible physiological role o f ecdysteroids in insect development have been briefly discussed. 
  Reference    Z. Naturforsch. 35c, 1066—1080 (1980); received June 19 1980 
  Published    1980 
  Keywords    Ecdysteroids, Juvenile Hormone, Metamorphosis, Dermestes maculatus 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-1066.pdf 
 Identifier    ZNC-1980-35c-1066 
 Volume    35 
5Author    U. Bührlen, H. Emmerich, H. RemboldRequires cookie*
 Title    Titer of Juvenile Hormone III in Drosophila hydei during Metamorphosis Determined by GC-MS-MIS  
 Abstract    The titer of juvenile hormone III (JH-III) has been determined by the use of combined gas chromatography-mass spectroscopy (GC-MS) in whole body extracts of the larvae, prepupae, pupae and adults of Drosophila hydei. A characteristic JH-III titer curve was established by use of the hormone derivatives. No other juvenile hormone homologs were detected. JH-III shows a broad peak maximum of about 30 pmol/g fresh weight during the last larval instar, whereas only traces of the hormone are detectable at pupariation. Prepupae and pupae exhibit about the same JH-III level. In older pupae and in the pharate adults there was no JH-III detectable but it reappeared soon after emergence. Low values of JH-III are found in young male and female flies. The JH contents rise to distinct peaks in older and reproductive adults, both in male and female animals. 
  Reference    Z. Naturforsch. 39c, 1150—1154 (1984); received June 29 1984 
  Published    1984 
  Keywords    Juvenile Hormone, Drosophila hydei, Metamorphosis, Reproduction, Gas Chromatography-Mass Spectroscopy 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-1150.pdf 
 Identifier    ZNC-1984-39c-1150 
 Volume    39 
6Author    Klaus Scheller, Peter Karlson, Dietrich BodensteinRequires cookie*
 Title    Effects of Ecdysterone and the Juvenile Hormone Analogue Methoprene on Protein, R N A and D N A Synthesis in Wing Discs of Calliphora vicina  
 Abstract    Adolf Butenandt zum 75. G ebu rtstag gew idm et The protein, RNA and DNA content of wing discs increase exponentially during the last larval instar. Biosynthesis of protein, RNA and DNA was studied by injecting labelled precursors into larvae or white prepupae and measuring the incorporation in the wing discs dissected out after an appropriate time. Protein synthesis is stimulated by ecdysterone, methoprene has no effect. Bio­ synthesis of rRNA is increased in wing discs of white prepupae after ecdysterone or methoprene injection. Methoprene inhibits the synthesis of mRNA, while ecdysterone has no clear-cut effect within the limits of our method (gel electrophoretic analysis). Ecdysterone and methoprene have no detectable influence on incorporation of thymidine into DNA, but the incorporation label from uridine into DNA is diminished; this effect may be due to changes in the precursor pool. 
  Reference    Z. Naturforsch. 33c, 253 (1978); received March 9 1978 
  Published    1978 
  Keywords    Ecdysterone, Juvenile Hormone, Wing Discs, Protein Synthesis, RNA Synthesis, DNA Synthesis, C alliph ora vicina 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0253.pdf 
 Identifier    ZNC-1978-33c-0253 
 Volume    33 
7Author    Hans-Peter Stupp, MartinG. PeterRequires cookie*
 Title    Juvenile Hormone III as a Natural Ligand for Photoaffinity Labelling of JH-Binding Proteins  
 Abstract    Juvenile hormone III, i.e. methyl 10,1 l-epoxy-3,7-dimethyl-2£',6£'-dodecadienoate, was used for photoaffinity labelling of JH-binding proteins. Irradiation of haemolymph from Manduca sexta and Locusta migratoria in the presence of racemic [10-3H]JH-III results in covalent 
  Reference    Z. Naturforsch. 39c, 1145—1149 (1984); received April 26/July 23 1984 
  Published    1984 
  Keywords    Insect Haemolymph, Hormone Binding Proteins, Juvenile Hormone, Photoaffinity Labelling, Manduca sexta, Locusta migratoria 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-1145.pdf 
 Identifier    ZNC-1984-39c-1145 
 Volume    39