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1Author    GeorgeP. Demagos, Wolfgang Baltus, Gerhard HöfleRequires cookie*
 Title    New Anthraquinone8 and Anthraquinone Glycosides from Morinda lucida  
 Abstract    From the trank heartwood of Morinda lucida four new anthraquinones, 3-hydroxy-anthraquinone-2-carbaldehyde, morindone-5-methyl ether, munjistin methyl ester, damna-canthol-cu-methyl ether and two glycosides, soranjidiol 6-0-/?-primveroside and lucidin 3-0-ß-primveroside were isolated and identified by spectroscopic and chemical methods. 
  Reference    Z. Naturforsch. 36b, 1180—1184 (1981); received May 20 1981 
  Published    1981 
  Keywords    Morinda lucida, Rubiaceae, Anthraquinones, Isolation 
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 TEI-XML for    default:Reihe_B/36/ZNB-1981-36b-1180.pdf 
 Identifier    ZNB-1981-36b-1180 
 Volume    36 
2Author    T. V. Rao, U. C. BiswalRequires cookie*
 Title    Isolation and Respiratory A ssay o f Earthworm Body W all M itochondria  
 Abstract    Out o f several media tested, KC1 m edium contain­ ing BSA was found to be most suitable for isolation o f functionally efficient mitochondria from earthworms. Respiration and oxidative phosphorylation in isolated mitochondria are demonstrated in the presence o f cyto­ chrome c and BSA in reaction medium. Introduction — The details o f the respiratory m etabolism in earthworm are yet to be established. There are only a few contradictory reports on the nature o f its respiratory enzyme components [1], W hile, cytochrome oxidase, suc-cinoxidase, succinate dehydrogenase were identified in earthworm tissue homogenates, the presence o f Kreb's cycle enzymes could not be demonstrated. Very little information is available on earthworm m itochondria except one report where it is isolated in 0.44 M m annitol [2]. Mitochondria from invertebrates like insects and crustaceans are isolated and characterized [3, 4]. But such methods cannot be extended to earthworms as it is known that conditions for isolation and assay o f m itochondria are highly system specific [5]. 
  Reference    Z. Naturforsch. 39c, 853 (1984); received January 23 1984 
  Published    1984 
  Keywords    Earthworm Mitochondria, Isolation, Respiratory Assay, Metabolism, Oxidative Phosphorylation 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0853_n.pdf 
 Identifier    ZNC-1984-39c-0853_n 
 Volume    39 
3Author    Ingrid Gilles, Hans-Gerd Löffler, Friedhelm SchneiderRequires cookie*
 Title    Ein neues Isolierungsverfahren für Schweinenierenacylase. Kinetik des Co2+, M n2+, N i2+ und Cd2+-enzyms A New Isolation Procedure for Acylamino Acid Amidohydrolase. Kinetics of the Co2+, M n2+, N i2+ and C d2+ Enzyme  
 Abstract    A new procedure for the isolation o f highly purified acylamino acid amidohydrolase from hog kidney is d e­ scribed which allows the preparation o f the enzym e with a recovery o f about 45%, a 200 fold purification and a spec.activity o f 3 5 0 -5 0 0 U. The essential Zn2+ o f the enzyme was exchanged for Co2+, N i2+, M n2+ and C d2+, and the kinetic parameters K M, kCdl and kCät/ K M o f the different enzyme species for a series o f acetyl-L-amino acids were determined. 
  Reference    Z. Naturforsch. 39c, 1017 (1984); received June 18/July 13 1984 
  Published    1984 
  Keywords    Acylamino Acid Amidohydrolase, Isolation, Metall Ion Exchange, Kinetics 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-1017_n.pdf 
 Identifier    ZNC-1984-39c-1017_n 
 Volume    39 
4Author    Manfred Wiegand, Karl EiseleRequires cookie*
 Title    Isolation and Characterization of the Androgen Receptor of Murine Preputial Gland  
 Abstract    The androgen receptor of murine preputial gland showed in binding experiments a biphasic saturation curve and a biphasic Scatchard plot. The receptor converted from an 8.5-9 S form to a 4.5—5 S form in high ionic strength buffer. The apparent dissociation constant was K D 0.5 ± 0.2 nM for the 8.5—9 S receptor form. A 6.5—7 S receptor form could be detected in some experiments. The ligand specificity was evaluated by competition experiments: testosterone > androstene-dione > dihydrotestosterone > androstanediol > estradiol > progesterone > dexamethasone. The receptor of murine preputial gland was less stable than the androgen receptor of skeletal muscle of the same mice. 
  Reference    Z. Naturforsch. 43c, 117—125 (1988); received September 25 1987 
  Published    1988 
  Keywords    Murine Preputial Gland, Androgen Receptor, Isolation, Characterization 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0117.pdf 
 Identifier    ZNC-1988-43c-0117 
 Volume    43 
5Author    W. Hülsen, D. KubinRequires cookie*
 Title    Isolierung und Eigenschaften der Zellhiille der Griinalge C h lo re lla f u s c a Isolation and Properties of the Cell Envelope of the Green Alga Chlorella fusca  
 Abstract    C hlorella cell envelopes were isolated by a mechanical procedure. The obtained morphological structure shown in an EM-picture has a mean density of .0 = 1.42. A charac­ teristic chemical property of the envelope is the percentage of mono-and di-unsaturated Cjg-fatty acids which is con­ siderably higher than that of intact cells. With 14C labelled sugars a selectivity of the hexose uptake could be shown, galactose uptake amounting only to about one fifth that of glucose and mannose. The uptake shows a temperature maximum. Part of the 14C registered after a 30 min uptake peroid is released by high concentration of unlabelled glu­ cose, 20% are irreversibly fixed. 
  Reference    (Z. Naturforsch. 30c, 684 [1975]; eingegangen am 25. Juni 1975) 
  Published    1975 
  Keywords    C hlorella, Cell Envelope, Isolation, Hexose Uptake, Fatty Acid Composition 
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 TEI-XML for    default:Reihe_C/30/ZNC-1975-30c-0684_n.pdf 
 Identifier    ZNC-1975-30c-0684_n 
 Volume    30 
6Author    W. Ilfried Brandi, KarlH. Errm An, LutzG. RotjahnRequires cookie*
 Title    Hydroxycinnamoyl Esters of Malic Acid in Small Radish {Raphanus sativus L. var. sativus)  
 Abstract    The esters o f /?-coumaric, ferulic and caffeic acid with m alic acid were isolated from leaves, the ester o f sinapic acid with m alic acid from cotyledons o f Raphanus sativus plants via prepara­ tive HPLC. The esters were identified by hydrolyses and follow ing capillary G C , by H -N M R and FAB-MS. Their concentrations in leaves and tubers were determ ined by gradient HPLC on reversed phase material. 
  Reference    Z. Naturforsch. 39c, 515 (1984); received March 6 1984 
  Published    1984 
  Keywords    Hydroxycinnamoylmalic Acids, Isolation, Identification, Q uantitative D eterm ination, Raphanus sativus 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0515.pdf 
 Identifier    ZNC-1984-39c-0515 
 Volume    39 
7Author    M. Ax Schönfelder, Ute Janott, Roland Frötschl, Karl-W Olfgang, M. Undry, G. Ünter, AdamRequires cookie*
 Title    Purification of Antiviral Proteins with Ribosome-Inactivating Properties from Plants  
 Abstract    A generally applicable highly effective purification method for antiviral plant proteins, selective for those with ribosome-inactivating properties, was developed. It involved affinity chromatography on Cibacron Blue Sepharose of the extracted plant sap after acid-ethanol precipitation and finally cation exchange chromatography on Mono S (Pharmacia). After the second chromatography step electrophoretically pure proteins from the four plant species, Chenopodium amaranticolor, Dianthus barbatus, Phytolacca americana and Spinacia oleracea, were obtained. These proteins inhibited infection of test plants with alfalfa mosaic virus and in vitro translation of TMV-RNA. The apparent molecular weights of the purified proteins were determined by SDS polyacrylamide gel electrophoresis as: 29 kDa for C. amaranticolor, 33 kDa for D. barbatus, 29 kDa for P. americana and 36 kDa for S. oleracea. Comparisons of the proteins by indirect ELISA and by Western blotting with polyclonal antisera from rabbits revealed that all four proteins were serologically related but not identical. In addition all four proteins were found to be glycosylated. 
  Reference    Z. Naturforsch. 47c, 731—738 (1992); received June 9/August 19 1992 
  Published    1992 
  Keywords    Antiviral Activity, Ribosome-Inactivating Proteins, Isolation, Serological Comparison, Glycosylation 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0731.pdf 
 Identifier    ZNC-1992-47c-0731 
 Volume    47 
8Author    P. V. Monje3, E. J. BaranbRequires cookie*
 Title    On the Formation of Whewellite in the Cactaceae Species Opuntia microdasys  
  Reference    Z. Naturforsch. 52c, 267—269 (1997); received January 20 1997 
  Published    1997 
  Keywords    Cactaceae, Opuntia microdasys, Whewellite, Isolation, Electron Microscopy, Infrared Spectrum 
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 TEI-XML for    default:Reihe_C/52/ZNC-1997-52c-0267_n.pdf 
 Identifier    ZNC-1997-52c-0267_n 
 Volume    52 
9Author    RichardP. Kreher, Gerald Dyker, WilhelmP. NeumannRequires cookie*
 Title    Structure and Reactivity of Isoannelated Heterocyclic Systems with Ann-and (4n+2)jr-Electrons, XII [1] 2-terr-Butyl-4-methyl-2,4-dihydropyrrolo[3,4-b]indoles: Tricyclic Hetarenes with Isoannelated Pyrrole Rings  
  Reference    Z. Naturforsch. 42b, 473—477 (1987); eingegangen am 14. November 1986 
  Published    1987 
  Keywords    Substituted 2, 3-Dihydropyrrolo[3, 4-b]indoles, Synthesis, Isolation, Spectroscopic Properties, Cycloaddition Reactions 
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 TEI-XML for    default:Reihe_B/42/ZNB-1987-42b-0473.pdf 
 Identifier    ZNB-1987-42b-0473 
 Volume    42 
10Author    RichardP. Kreher, Thomas HildebrandRequires cookie*
 Title    Structure and Reactivity of Isoannellated Heterocyclic Systems with 4nji-and (4/7+2);r-Electrons, XIV [1] Tri-and Tetracyclic Hetarenes with Localized or Delocalized Pyrrole Units  
 Abstract    2,7-Di-/er/-butyl-2,7-dihydro-benzo[ 1,2-c:3,4-c']dipyrrole (2b) and 2,5,8-Tri-rm-butyl-5.8-di-hydro-2H-benzo[l,2-c:3,4-c':5,6-c"]tripyrrole (3b) have been prepared by acetolysis of the cor-responding tri-and tetracyclic N-oxides, isolated in crystallinic form and characterized by spec-troscopic data. The scope of the N-oxide route is documented and the existence of benzo-annellated pyrroles is proofed. The stabilizing effect of the tert-butyl group is presumably sup-ported by the vicinal annellated heterocycles. 
  Reference    (Z. Naturforsch. 43b, 125—131 [1988]; eingegangen am 15. September 1987) 
  Published    1988 
  Keywords    Benzo-annellated Di-and Tripyrroles, Synthesis, Isolation, Spectroscopic Properties, Cycloaddition Reactions 
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 TEI-XML for    default:Reihe_B/43/ZNB-1988-43b-0125.pdf 
 Identifier    ZNB-1988-43b-0125 
 Volume    43 
11Author    Susanne Valcic3, Gloria Montenegro15, Ana-Maria Mujicab, Guacolda Avilab, Scott Franzblauc, MayaP. Singhd, WilliamM. Maiesed, BarbaraN. Timmermann3Requires cookie*
 Title    Phytochemical, Morphological, and Biological Investigations of Propolis from Central Chile  
 Abstract    Propolis from Central Chile was investigated for its plant origin by microscopical analysis of pollen grains and leaf fragments found in the sample. The pollen grains that appear with significant higher frequency in the sample corresponded to four native and two introduced species, whereas leaf fragments corresponded to four native species. Seventeen phenolic com­ pounds that belong to the phenylpropane, benzaldehyde, dihydrobenzofuran, or benzopyran classes, were isolated from an organic extract that was found to have a moderate growth inhibitory activity against M ycobacterium avium , M. tuberculosis, and two strains of S taphylo­ coccus aureus. The components responsible for activity were determined. 
  Reference    Z. Naturforsch. 54c, 406 (1999); received October 29 1998/February 15 1999 
  Published    1999 
  Keywords    Propolis, Central Chile, Microscopical Analysis, Isolation, Phenylpropanes, Dihydrobenzofurans, Benzopyrans, Antimicrobial Activity 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-0406.pdf 
 Identifier    ZNC-1999-54c-0406 
 Volume    54