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1996 (1)
1Author    Grzegorz JackowskiRequires cookie*
 Title    Senescence-Related Changes in the Subcomplex Arrangement of the Major Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II (LHCII) as Influenced by Cytokinin  
 Abstract    The major light-harvesting chlorophyll a /b -protein complex of photosystem II (LHCII) from fresh barley leaves could be resolved by non-denaturing IEF into five trimeric subcom­ plexes designated 1 -5 in order of decrasing pi value. IEF-based analysis of PSII particles isolated from leaves in which the processes of senescence were induced by detachement and dark-incubation in the presence of water for 0 -8 days let us reveal that substantial rearrangements of LHCII organization took place throughout the course of senescence com ­ prising a step-wise decline in relative abundance of subcom plexes 1 -3 (down to 0-58% of the initial abundance during 8 days of aging) and an increase in the relative abundance of the subcom plexes 4 and 5. Using SDS-PAGE and immunoblot analysis it was shown that the rearrangements were linked to the changes in the relative levels of LHCII apoproteins i.e. 26.7 and 25.6 kDa ones. The changes comprised the preferential disappearance of the 26.7 kDa polypeptide and an enrichment of 25.6 kD a one and most probably reflect the hetero­ geneity among LHCII apoproteins concerning their stability under the conditions of chi loss. Kinetin was able to repress the senescence-related rearrangements in LHCII subcomplex organization at late stages of aging (5 -8 days) by preventing over this time period the disap­ pearance of 26.7 kD a polypeptide and the enrichement of 25.6 kDa one. 
  Reference    Z. Naturforsch. 51c, 464 (1996); received Novem ber 23 1995/ 
  Published    1996 
  Keywords    Aging, Barley, Isoelectric Focusing, Polypeptide, Rearrangement 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0464.pdf 
 Identifier    ZNC-1996-51c-0464 
 Volume    51