| 3 | Author
| G. Ünther, F. E. Scherer | Requires cookie* | | Title
| A New M ethod to Prepare M em brane F ractions Containing Ionophore-Stim ulated A T P a se from Pumpkin H ypocotyls (Cucurbita maxima, L .)  | | | Abstract
| In membrane fractions from pumpkin hypocotyls AT Pase activity was stimulated by a combination o f CCCP (carbonyl cyanide m-chlorophenylhydrazone), a pro-tonophore, and valinomycin, a K +-ionophore. Singly, these ionophores stimulated ATPase activity much less. Nigericin, an H+/K +-antiporter, and nystatin, a cation pore, had similar effects as the combination o f CCCP and valinomycin. The results suggest the presence o f a cation-translocating ATPase which is stimulated by ionophores by dissipating cation gradients formed in the vesicles. A major part of the ionophore-stimulated ATPase activity correlat ed with marker enzymes for plasma membranes but part o f it could be located in other compartments. | | |
Reference
| Z. Naturforsch. 37c, 550 (1982); received March 5 1982 | | |
Published
| 1982 | | |
Keywords
| H +-ATPase, Ionophores, Plasma Membrane, Cucurbita | | |
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| default:Reihe_C/37/ZNC-1982-37c-0550_n.pdf | | | Identifier
| ZNC-1982-37c-0550_n | | | Volume
| 37 | |
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