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1980 (1)
1Author    ThomasL J Boehm, Dusan DrahovskyRequires cookie*
 Title    Die Beziehung der enzymatischen Methylierung invers repetitiver DNA Sequenzen zur Transkription in Maus P815 Mastocytoma Zellen The Relation o f Enzymatic M ethylation of Inverted DNA Repeats to Transcription in Mouse P815 M astocytoma Cells  
 Abstract    The isolation of transcribed D NA sequences of P815 cells and the partial characterization with respect to their sequence composition and relative rates o f enzymatic D NA m ethylation are reported in this paper. Transcribed regions were purified by affinity chrom atography using im ­ mobilized heterogenous nuclear RNA of P815 cells. About 10% of total genome was found in this fraction. Reassociation analyses showed differences in sequence composition o f transcribed versus non-transcribed D N A fractions. The relative proportion of inverted repeats was doubled in the transcribed fraction whereas ordinary highly repetitive sequences comprising mainly o f satellite D NA were found alm ost exclusively in the non-transcribed regions o f the P815 genome. A bout 70% of transcribed portions corresponds to unique and interm ediary D NA sequences. A fter labelling of cells with L-[Methyl-3H]methionine and [14C]deoxycytidine relative rates o f enzymatic DNA m ethylation were computed for different kinetic components o f transcribed and non-transcribed portions of P815 genome. No difference was found except in inverted repeats. In transcribed D NA the relative rate o f enzymatic DNA methylation was only about 40% o f that of the non-transcribed ones. We have quantitated this hypomethylation and found that there is, in average, about one 5-methylcytosine residue in 100 nucleotides o f transcribed inverted repeats, com pared to about 2.5 5-methylcytosines in non-transcribed fractions. In view of these data we propose that the enzymatic methylation o f inverted DNA repeats negatively controls the tran ­ scriptional process in a given genomic region. 
  Reference    Z. Naturforsch. 35c, 611—6 (1980); eingegangen am 30. Januar 1980 
  Published    1980 
  Keywords    Enzymatic Methylation, Inverted D NA Repeats, Transcriptional Control 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0611.pdf 
 Identifier    ZNC-1980-35c-0611 
 Volume    35