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1978 (1)
1Author    Satham Saphon, Peter GräberRequires cookie*
 Title    External Proton Uptake, Internal Proton Release and Internal pH Changes in Chromatophores from Rps. sphaeroides Following Single Turnover Flashes  
 Abstract    The change in internal pH in chromatophores from R ps. sph aeroides following short flash ex­ citation has been investigated using the method of the fluorescence quenching of 9-aminoacridine. (1) A single turnover flash induced a fluorescence quenching of ^ 0.4%. The fluorescence quenching was stim ulated by valinomycin, and oligomycin, and inhibited by antim ycin A. (2) The extent of the pH gradient generated by a single turnover flash at the external pH ^ 8 was estim ated to be less than 0.4 unit. (3) In a multiflash experiment the change in internal pH induced by each flash was found to be not linearly dependent on the num ber of protons which had been taken up after each flash from the external medium. We discuss the significance of the results w ith respect to the mechanism of phosphorylation. The release of H+ taken up outside the chromatophore into the m em brane could not be excluded by the present results. 
  Reference    Z. Naturforsch. 33c, 715—722 (1978); received July 31 1978 
  Published    1978 
  Keywords    Proton Translocation, Internal pH-Changes, Phosphorylation 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0715.pdf 
 Identifier    ZNC-1978-33c-0715 
 Volume    33