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1	Author	M. Valentin, W. E. Bollenbacher, L. I. Gilbert, H. Kroeger	Requires cookie*
	Title	Alterations in Ecdysone Content during the Post-Embryonic Development of Chironomus thummi: Correlations with Chromosomal Puffing
	Abstract	The ecdysone titer o f larvae and pupae o f Chironomus thum m i was determined by radioimmune assay (R IA) and revealed a concentration of about 150 ng/g fresh weigth in late third instar larvae and a peak of more than 450 ng/g fresh weight just preceding pupation. The ecdysone titer curve shows a high degree o f correlation with previously observed puffing activity at the ecdysone sensitive chromosomal site I l l d l . Further, //-ecdysone is the only endogenous ecdysone detected by high pressure liquid chromatography during larval-pupal development. It was also observed that developmentally arrested Chironomus contain less ecdysone than " normal" larvae.
	Reference	Z. Naturforsch. 33c, 557 (1978); received June 5 1978
	Published	1978
	Keywords	Insect Hormones, Ecdysteroids, Chromosomal Puffing, Chironomus thum mi
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	TEI-XML for	default:Reihe_C/33/ZNC-1978-33c-0557.pdf
	Identifier	ZNC-1978-33c-0557
	Volume	33

2	Author	Peter Mascheck, Klaus Scheller, Peter Karlson	Requires cookie*
	Title	Changes in Gene Expression during Larval Development of Calliphora vicina Induced by Ecdysterone
	Abstract	The influence of the insect hormone ecdysterone on the composition of the RNA from the fat bodies of C alliph ora larvae was investigated by hybridization experiments. DNA was iodinated in vitro with 125J. In saturation experiments, total RNA was hybridized to total DNA; under similar conditions, poly (A) RNA was hybridized to unique DNA sequences. RNA isolated from animals treated with ecdysterone exhibits a higher percentage of hybridization than a corresponding control RNA from the same developmental stage, a result even more prominent when poly (A) RNA was hybridized with unique DNA. It is concluded that ecdysterone induces the transcription of new unique DNA sequences.
	Reference	(Z. Naturforsch. 32c, 434 [1977]; received December 27 1976)
	Published	1977
	Keywords	Gene Expression, Insect Hormones, Ecdysterone, Larval Development, DNA-RNA Hybridization
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	TEI-XML for	default:Reihe_C/32/ZNC-1977-32c-0434.pdf
	Identifier	ZNC-1977-32c-0434
	Volume	32