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1978 (1)
1977 (1)
1Author    M. Valentin, W. E. Bollenbacher, L. I. Gilbert, H. KroegerRequires cookie*
 Title    Alterations in Ecdysone Content during the Post-Embryonic Development of Chironomus thummi: Correlations with Chromosomal Puffing  
 Abstract    The ecdysone titer o f larvae and pupae o f Chironomus thum m i was determined by radioimmune assay (R IA) and revealed a concentration of about 150 ng/g fresh weigth in late third instar larvae and a peak of more than 450 ng/g fresh weight just preceding pupation. The ecdysone titer curve shows a high degree o f correlation with previously observed puffing activity at the ecdysone sensitive chromosomal site I l l d l . Further, //-ecdysone is the only endogenous ecdysone detected by high pressure liquid chromatography during larval-pupal development. It was also observed that developmentally arrested Chironomus contain less ecdysone than " normal" larvae. 
  Reference    Z. Naturforsch. 33c, 557 (1978); received June 5 1978 
  Published    1978 
  Keywords    Insect Hormones, Ecdysteroids, Chromosomal Puffing, Chironomus thum mi 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0557.pdf 
 Identifier    ZNC-1978-33c-0557 
 Volume    33 
2Author    Peter Mascheck, Klaus Scheller, Peter KarlsonRequires cookie*
 Title    Changes in Gene Expression during Larval Development of Calliphora vicina Induced by Ecdysterone  
 Abstract    The influence of the insect hormone ecdysterone on the composition of the RNA from the fat bodies of C alliph ora larvae was investigated by hybridization experiments. DNA was iodinated in vitro with 125J. In saturation experiments, total RNA was hybridized to total DNA; under similar conditions, poly (A) RNA was hybridized to unique DNA sequences. RNA isolated from animals treated with ecdysterone exhibits a higher percentage of hybridization than a corresponding control RNA from the same developmental stage, a result even more prominent when poly (A) RNA was hybridized with unique DNA. It is concluded that ecdysterone induces the transcription of new unique DNA sequences. 
  Reference    (Z. Naturforsch. 32c, 434 [1977]; received December 27 1976) 
  Published    1977 
  Keywords    Gene Expression, Insect Hormones, Ecdysterone, Larval Development, DNA-RNA Hybridization 
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 TEI-XML for    default:Reihe_C/32/ZNC-1977-32c-0434.pdf 
 Identifier    ZNC-1977-32c-0434 
 Volume    32