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1989 (1)
1982 (1)
1Author    Angela De, Souza Otero, Leopoldo De MeisRequires cookie*
 Title    Phosphorylation o f Ca2+-ATPase by Inorganic Phosphate in Water-Organic Solvent Media: Dielectric Constant and Solvent Hydrophobicity Contribution1  
 Abstract    The effect o f organic solvents on the phosphorylation o f the Ca2+-dependent ATPase o f sar­ coplasmic reticulum by inorganic phosphate in the absence o f a calcium gradient was investi­ gated. Kinetic analysis o f the reaction in water and water-organic solvent m edia according to a bireactant scheme shows no correlation betw een changes in kinetic parameters and the dieletric constant o f the mixed solvents. The pronounced increase in equilibrium levels o f phosphoenzym e in water-solvent mixtures is attributed to changes in the water activity o f the m edium. 
  Reference    Z. Naturforsch. 37c, 527 (1982); received January 4 1982 
  Published    1982 
  Keywords    Phosphorylation, Ca2+-ATPase, Inorganic Phosphate, O rganic Solvents 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0527.pdf 
 Identifier    ZNC-1982-37c-0527 
 Volume    37 
2Author    Kaoru Kubota, Xiao-Ni Li1, Hiroshi AshiharaRequires cookie*
 Title    The Short-Term Effects of Inorganic Phosphate on the Levels of Metabolites in Suspension-Cultured Catharanthus roseus Cells  
 Abstract    In order to analyze the effects o f inorganic phosphate (Pi) on the energy metabolism o f suspen­ sion-cultured cells o f Catharanthus roseus, short-term changes in levels o f glycolytic m etabolites were m onitored after the addition o f 1.25 mM Pi to cultures of cells previously cultured in a Pi-free M urashige-Skoog m edium for 24 h. The levels o f all phosphorylated interm ediates o f glycolysis exam ined were found to increase after addition o f Pi. The most striking increases were observed in levels o f glucose-6-phosphate and fructose-6-phosphate which rose to approximately 8 (after 30 min) and 15 tim es (after 120 min) their initial values. The levels o f A TP and pyrophosphate doubled during the first 30 min o f incubation. In contrast, the level of pyruvate decreased signifi­ cantly during the initial 30 min and then began to increase again for the next 90 min. The short­ term effects o f Pi on the rates o f synthesis o f proteins and R N A were estim ated from the rates o f incorporation o f 14C-labelled am ino acids and uridine into the macrom olecules. No appreciable stim ulation o f the synthesis o f proteins and R N A was induced by Pi within 120 min after the addition o f Pi. Possible m echanism s are discussed that may be involved in the changes in m etab o­ lism initiated by the addition o f Pi to the cultures. 
  Reference    Z. Naturforsch. 44c, 802—806 (1989); received June 5 1989 
  Published    1989 
  Keywords    Catharanthus roseus, Suspension Culture, Inorganic Phosphate, G lycolytic M etabolites, M etabolic Regulation 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0802.pdf 
 Identifier    ZNC-1989-44c-0802 
 Volume    44