| | 1 | Author
| Renate Horcher, John Wilcockson, D. Ietrich, W. Erner | Requires cookie* | | | Title
| Screening for Mutants of Rhizobium japonicum with Defects in Nitrogen Fixing Ability  | | | | Abstract
| Mutants o f Rhizobium japonicum with reduced ex planta nitrogenase activity could be isolated with high frequency by direct screening of ultra-violet mutagenized bacteria growing as spots on the surface o f an appropriate agar medium permitting derepression of nitrogenase synthesis. Small glass chambers fitted with a serum cap were pushed into the agar around each spot of growth, forming a small enclosed gas space which was made to 10% acetylene, permitting assessment o f nitrogenase activity by the acetylene reduction test. Four mutants were isolated from a total of 305 screened spots. Two mutants had almost no ex planta activity, one o f which had no symbiotic activity despite normal nodulation (ineffective), the other had only somewhat reduced activity symbiotically. Two other mutants with less than half wild-type activity ex planta were normal in symbiosis. | | | |
Reference
| Z. Naturforsch. 35c, 729—732 (1980); received May 30 1980 | | | |
Published
| 1980 | | | |
Keywords
| Rhizobium japonicum, Nitrogenase, Nif-Mutants, Ineffective Nodules | | | |
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| default:Reihe_C/35/ZNC-1980-35c-0729.pdf | | | | Identifier
| ZNC-1980-35c-0729 | | | | Volume
| 35 | |
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