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'Immunofluorescence' in keywords
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1997 (1)
1980 (1)
1Author    Klaus Scheller, Hans-Peter Zim, Erm Ann, ConstantinE. SekerisRequires cookie*
 Title    Calliphorin, a Protein Involved in the Cuticle Formation of the Blowfly, Calliphora vicina  
 Abstract    Injections of radioactively labelled calliphorin into Illrd instar larvae o f Calliphora indicate that the protein is incorporated into the integument. Immunofluorescence techniques show that the endocuticle is the Final site o f deposition o f calliphorin. It is concluded that calliphorin is an essen­ tial protein of the tanned cuticle. 
  Reference    Z. Naturforsch. 35c, 387—389 (1980); received December 28 1979/February 14 1980 
  Published    1980 
  Keywords    Calliphorin, Insect Cuticle, Immunofluorescence, Calliphora vicina 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0387.pdf 
 Identifier    ZNC-1980-35c-0387 
 Volume    35 
2Author    K. Künzler3, W. Eichenberger3, A. R. AdunzbRequires cookie*
 Title    Intracellular Localization of Two Betaine Lipids by Cell Fractionation and Immunomicroscopy  
 Abstract    The cellular localization of the betaine lipids diacylglyceryl-N,/V,/V-trimethylhomoserine (D G TS) and diacylglycerylhydroxymethyl-/V,./V,/V-trimethyl-ß-alanine (D G T A) was investi­ gated by a) chemical analysis of subcellular fractions and b) immunochemical methods using specific antisera and either fluorescence microscopy or electron microscopy for detection of the label. A homogenate of L ycopodium annotinum (Pteridophyta) was fractionated by differential and density gradient centrifugation. The particulate fractions obtained were ana­ lyzed for chlorophyll, cyt c oxidase, N A D H -cyt c reductase and DGTS. Non-plastidial frac­ tions were enriched in DGTS and only minor amounts o f this lipid could be attributed to chloroplasts. Anti-DGTS and anti-DGTA sera were produced by immunization of rabbits. The monospecificity of the antisera was examined with cells of C hlam ydom onas reinhardtii (Chlorophyceae) containing DGTS, Pavlova lutheri (H aptophyceae) containing DGTA and Ochromonas danica (Chrysophyceae) containing both DGTS and DGTA. Euglena gracilis which is free of betaine lipids, was used as a control. For the test, a FITC-coupled goat anti-rabbit antibody was used and detected by fluorescence microscopy. Thin sections of Ochromonas and Pavlova were incubated first with the anti-lipid sera and subsequently with a gold-coupled anti-rabbit serum and then examined in the electron microscope. With O chro­ monas, anti-DGTS as well as anti-DGTA sera gave an accumulation of gold label in the cytoplasmic space but not in the chloroplasts. Similar results were obtained with Pavlova using anti-DGTA serum. These results describe for the first time the cytochemical localiza­ tion of DGTS and DGTA strongly suggesting both these lipids to be associated mainly with non-plastidial structures. 
  Reference    Z. Naturforsch. 52c, 487—4 (1997); received March 28/May 12 1997 
  Published    1997 
  Keywords    Antibodies, D G TA, DGTS, Immunoelectron Microscopy, Immunofluorescence, Membrane Fractions 
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 TEI-XML for    default:Reihe_C/52/ZNC-1997-52c-0487.pdf 
 Identifier    ZNC-1997-52c-0487 
 Volume    52