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'Immobilization' in keywords Facet   section ZfN Section C  [X]
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1Author    I. F. Kaetsu, T. Yoshii, FujimuraRequires cookie*
 Title    Effect of Viscous Solvents and Monomer on Conservation of Intact and Immobilized Chloroplasts  
 Abstract    0 2 evolution activity of PS II in chloroplast stored in buffer including various supercooling solvents was studied. Viscous solvents such as polyethylene glycol (PEG) was remarkably effective for the increase o f lifetime o f intact chloroplast. Lifetime of immobilized chloroplast by means of radiation polymerization was prolonged more than 40 days in buffer including PEG 70% (v/v). It was found that viscous solvents having PEG units in molecular chain were effective and methoxypolyethyleneglycol methacrylate monomer (M-23G) was most suitable as a im­ mobilization carrier. The immobilized chloroplast with M-23G retained the high activity yield more than 30 days in buffer including PEG. 
  Reference    Z. Naturforsch. 35c, 1052—1056 (1980); received September 2 1980 
  Published    1980 
  Keywords    Chloroplast, Immobilization, Oxygen Evolution, Viscous Solvent, Radiation Polymerization 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-1052.pdf 
 Identifier    ZNC-1980-35c-1052 
 Volume    35 
2Author    Ernst Bayer, Heribert Hellstern, Heiner EcksteinRequires cookie*
 Title    Synthese von immobilisierten Peptidfragmenten an Polystyrol-Polyoxyethylen zur Affinitätschromatographie Synthesis of Immobilized Peptide Fragments on Polystyrene-Polyoxyethylene for Affinity Chromatography  
 Abstract    Polystyrene-polyoxyethylene craft copolymers have been used for step-wise peptide synthesis. After completion of synthesis the protecting groups are cleaved under acidic conditions, where the polymer-peptide bond is stable. These gels in comparison to polystyrene peptide gels, show better properties for applications in affinity chromatography as well as synthesis on solid supports, because the advantageous properties of polystyrene beads are combined with the excellent spacer behavior of polyoxyethylene chains (mobility, solvation by water and organic solvents). Peptide gels with polylysine sequences have been synthesized as highly selective stationary phases for the separation of the homologous oligo desoxyribonucleotides (d7)n with n = 1—3. The principal possibilities of these gels for affinity chromatography 
  Reference    Z. Naturforsch. 42c, 455—460 (1987); received December 1 1986 
  Published    1987 
  Keywords    birthday Affinity Chromatography, Immobilization, Liquid-Solid-Phase, Peptides, Synthesis 
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 TEI-XML for    default:Reihe_C/42/ZNC-1987-42c-0455.pdf 
 Identifier    ZNC-1987-42c-0455 
 Volume    42 
3Author    Günter Pilwat, Peter Washausen, Joachim Klein, Ulrich ZimmermannRequires cookie*
 Title    Immobilization of Human Red Blood Cells  
 Abstract    H uman red blood cells were immobilized in an alginate network which was cross-linked with C a2+ ions. The im ­ mobilized cells were stored for longer than 5 weeks at 4 °C in an isotonic buffered NaCl solution containing glucose, inosine, adenine, and guanosine for energy supply. The im ­ mobilized cells were released from the alginate network by dissolving the matrix with citrate. Both the im mobilized and released cells retain their biconcave shape over the storage period. Measurements of the released cell popula­ tion in a hydrodynamically focussing Coulter C ounter demonstrated that the mean size of the size distribution, the breakdown voltage, and the internal conductivity have not changed in contrast to control m easurem ents on red blood cells stored conventionally in suspension indicating that im mobilization preserves cellular functions. 
  Reference    Z. Naturforsch. 35c, 352—356 (1980); received December 4 1979 
  Published    1980 
  Keywords    Red Blood Cells, Immobilization, Reversible Gels, Electri­ cal Breakdown, Coulter Counter 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0352_n.pdf 
 Identifier    ZNC-1980-35c-0352_n 
 Volume    35 
4Author    T. Fujimura, I. KaetsuRequires cookie*
 Title    Immobilization of Yeast Cells by Radiation-Induced Polymerization  
 Abstract    R adiation-induced polym erization m ethod was applied to the im m obilization of yeast cells. The effects of irradiation, cooling and monom er, which are neccessary for polymerization, were recovered completely by subsequent aerobical incubation of yest cells. The ethanol productive in immobilized yeast cells increased with the increase o f aerobical incubation period. The growth of yeast cells in im m obilized yeast cells was indicated. The m axim um ethanol productivity in immobilized yeast cell system was around three times as much as that in free yeast cell system. 
  Reference    Z. Naturforsch. 37c, 102 (1982); received Septem ber 111981 
  Published    1982 
  Keywords    Yeast, Immobilization, R adiation Polymerization, R adiation Effect, Ethanol Production 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0102.pdf 
 Identifier    ZNC-1982-37c-0102 
 Volume    37 
5Author    T. Fujim, F. Yoshii, I. Kaetsu, Y. Inoue, K. ShibataRequires cookie*
 Title    Effect of Irradiation and Immobilization on Spinach Chloroplast Activities  
 Abstract    The effect of y-ray irradiation and immobilization by means of radiation polymerization on PS II activity (0 2 evolution) o f isolated chloroplasts from spinach was investigated. Reduction o f 0 2 evolution activity by irradiation was small at lower temperatures below — 24 °C, but the activity decreased slightly by freezing at extremely low temperature below —78 °C. So the optimum low temperature range for the treatment was observed. The immobilized chloroplast in a hydrophilic polymer matrix showed the stable duration o f 0 2 evolution activity more than 700 h at 4 °C . Thermo-stability o f chloroplast was also improved greatly by immobilization. The active center o f PS II in immobilized chloroplasts was retained even after 60 min standing at 50 °C. 
  Reference    Z. Naturforsch. 35c, 477—481 (1980); received February 12 1980 
  Published    1980 
  Keywords    Radiation Effect, Chloroplast, 0 2 Evolution, Immobilization, Radiation Polymerization, Low Temperatures 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0477.pdf 
 Identifier    ZNC-1980-35c-0477 
 Volume    35 
6Author    Fumio Yoshii, Isao Kaetsu, Akio YamadaRequires cookie*
 Title    Immobilization of Erythrocytes by Radiation Polymerization of Glass-Forming Monomers at Low Temperatures  
 Abstract    The immobilization of erythrocyte as the whole cell without hemolysis was studied. It found that erythrocyte could be treated and immobilized stably by radiation polymerization o f specific monomers having high viscous and long oxyethylene units chain such as methoxypolyethylene-glycol methacrylate (M-23G) and polyethyleneglycol dimethacrylate (14G). Irradiation dose without hemolysis was limited less than lxl05r and a comonomer system consisting o f M -23G-14G, 1:1 and small quantity o f glutaraldehyde (GA) was the optimum carrier composition. The functional properties o f the immobilized erythrocyte was also investigated. It was found that the immobilized cell could be carried out carbon monoxide-oxygen gas exchange effectively and reversibly so as in the intact cell. The immobilized erythrocyte also showed the catalase activity just as in the intact cell. The stability o f erythrocyte increased greatly by the immobilization for standing at low and room temperatures and hardly hemolyzed in non-isotonic medium such as pure water. It was observed in scanning electron microscope that the immobilized erythrocyte had a hollow disk shape same as in intact cell and covered with a thin polymer layer. 
  Reference    Z. Naturforsch. 36c, 1062—1067 (1981); received June 2 1981 
  Published    1981 
  Keywords    Erythrocyte, Radiation Polymerization, Immobilization, Glass-Forming Monomer, Low Temperature, 0 2-Uptake 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-1062.pdf 
 Identifier    ZNC-1981-36c-1062 
 Volume    36