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'Immobilization' in keywords Facet   Publication Year 1980  [X]
Facet   section ZfN Section C  [X]
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1Author    I. F. Kaetsu, T. Yoshii, FujimuraRequires cookie*
 Title    Effect of Viscous Solvents and Monomer on Conservation of Intact and Immobilized Chloroplasts  
 Abstract    0 2 evolution activity of PS II in chloroplast stored in buffer including various supercooling solvents was studied. Viscous solvents such as polyethylene glycol (PEG) was remarkably effective for the increase o f lifetime o f intact chloroplast. Lifetime of immobilized chloroplast by means of radiation polymerization was prolonged more than 40 days in buffer including PEG 70% (v/v). It was found that viscous solvents having PEG units in molecular chain were effective and methoxypolyethyleneglycol methacrylate monomer (M-23G) was most suitable as a im­ mobilization carrier. The immobilized chloroplast with M-23G retained the high activity yield more than 30 days in buffer including PEG. 
  Reference    Z. Naturforsch. 35c, 1052—1056 (1980); received September 2 1980 
  Published    1980 
  Keywords    Chloroplast, Immobilization, Oxygen Evolution, Viscous Solvent, Radiation Polymerization 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-1052.pdf 
 Identifier    ZNC-1980-35c-1052 
 Volume    35 
2Author    Günter Pilwat, Peter Washausen, Joachim Klein, Ulrich ZimmermannRequires cookie*
 Title    Immobilization of Human Red Blood Cells  
 Abstract    H uman red blood cells were immobilized in an alginate network which was cross-linked with C a2+ ions. The im ­ mobilized cells were stored for longer than 5 weeks at 4 °C in an isotonic buffered NaCl solution containing glucose, inosine, adenine, and guanosine for energy supply. The im ­ mobilized cells were released from the alginate network by dissolving the matrix with citrate. Both the im mobilized and released cells retain their biconcave shape over the storage period. Measurements of the released cell popula­ tion in a hydrodynamically focussing Coulter C ounter demonstrated that the mean size of the size distribution, the breakdown voltage, and the internal conductivity have not changed in contrast to control m easurem ents on red blood cells stored conventionally in suspension indicating that im mobilization preserves cellular functions. 
  Reference    Z. Naturforsch. 35c, 352—356 (1980); received December 4 1979 
  Published    1980 
  Keywords    Red Blood Cells, Immobilization, Reversible Gels, Electri­ cal Breakdown, Coulter Counter 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0352_n.pdf 
 Identifier    ZNC-1980-35c-0352_n 
 Volume    35 
3Author    T. Fujim, F. Yoshii, I. Kaetsu, Y. Inoue, K. ShibataRequires cookie*
 Title    Effect of Irradiation and Immobilization on Spinach Chloroplast Activities  
 Abstract    The effect of y-ray irradiation and immobilization by means of radiation polymerization on PS II activity (0 2 evolution) o f isolated chloroplasts from spinach was investigated. Reduction o f 0 2 evolution activity by irradiation was small at lower temperatures below — 24 °C, but the activity decreased slightly by freezing at extremely low temperature below —78 °C. So the optimum low temperature range for the treatment was observed. The immobilized chloroplast in a hydrophilic polymer matrix showed the stable duration o f 0 2 evolution activity more than 700 h at 4 °C . Thermo-stability o f chloroplast was also improved greatly by immobilization. The active center o f PS II in immobilized chloroplasts was retained even after 60 min standing at 50 °C. 
  Reference    Z. Naturforsch. 35c, 477—481 (1980); received February 12 1980 
  Published    1980 
  Keywords    Radiation Effect, Chloroplast, 0 2 Evolution, Immobilization, Radiation Polymerization, Low Temperatures 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0477.pdf 
 Identifier    ZNC-1980-35c-0477 
 Volume    35