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'Hydroxycinnamic Acid' in keywords Facet   Publication Year 1980  [X]
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1980[X]
1Author    A. Fleuriet, J. J. Macheix, R. Suen, R. K. IbrahimRequires cookie*
 Title    Partial Purification and Some Properties of a Hydroxycinnamoyl Glucosyltransferase from Tomato Fruits  
 Abstract    A glucosyltransferase was isolated from im mature "cherry" tom atoes and was partially purified (200-fold) by am m onium sulphate precipitation and successive chrom atography on Sephadex G-100 and DEAE-cellulose columns. The enzyme utilised the free hydroxycinnamic acids and UDP-glucose in the form ation o f their respective glucosides (pH 8.0) and glucose esters (pH 7.0); but did not accept the CoA thiolesters o f HCAs in the presence of glucose-1-phosphate. The constant glucoside/glucose ester ratio observed during purification suggests that both reactions are catalysed by the sam e enzyme. The K m values for /»-coumaric, caffeic, ferulic and sinapic acids were 0.8, 1.5, 1.4 and 2.5 hm, respectively. W ith ferulic acid as substrate, the K m value for U D PG was 10 hm. The enzyme required an -S H group for activity and the reaction was strongly inhibited by EDTA, divalent metal ions and UDP. 
  Reference    Z. Naturforsch. 35c, 967—9 (1980); received August 14 1980 
  Published    1980 
  Keywords    Glucosyltransferase, Glucosides, Glucose Esters, Hydroxycinnamic Acids, Enzyme Purification and Properties, Tom ato Fruit, Lycopersicum esculentum (Solanaceae) 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0967.pdf 
 Identifier    ZNC-1980-35c-0967 
 Volume    35