| | 1 | Author
| Kenji Matsui, Yasushi Shibata, Tadahiko Kajiwara, AkikazuH. Atanaka | Requires cookie* | | | Title
| Separation of 13-and 9-Hydroperoxide Lyase Activities in Cotyledons of Cucumber Seedlings  | | | | Abstract
| In cucumber cotyledons, both C6-and C9-aldehyde were formed via hydroperoxide (H PO) lyase activity. Because it has not been elucidated whether these activities are attri buted to one enzym e which can cleave both 13-and 9-H PO or to two or more enzym es each o f which specifically cleaves 13-or 9-H PO , an attempt to separate HPO lyase activity was done. Ion exchange chromatography separated this activity into two fractions, one o f which specifically cleaved 13-hydroperoxylinoleic acid and the other specifi cally cleaved the 9-isomer. 13-H PO -specific activity was most active at pH 8.0 and 9-H PO -specific one was at pH 6.5. SH -reagents inhibited both the lyases but to differ ent extents. | | | |
Reference
| Z. Naturforsch. 44c, 883—885 (1989); received April 251989 | | | |
Published
| 1989 | | | |
Keywords
| Cucumber Cotyledons, Hydroperoxide Lyase, Fatty A cid 13-H ydroperoxide, Fatty Acid 9-H ydroperoxide, Short-Chain A ldehydes | | | |
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| ZNC-1989-44c-0883_n | | | | Volume
| 44 | |
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