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1993 (2)
1981 (1)
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1977 (1)
1Author    Lester Packer, William CullingfordRequires cookie*
 Title    Stoichiometry of H 2 Production by an in vitro Chloroplast, Ferredoxin, Hydrogenase Reconstituted System  
 Abstract    Studies of the rate and efficiency of H 2 production by a reconstituted system composed of washed chloroplasts, ferredoxin, and hydrogenase reveal th at a significant proportion of the low potential electrons are leaked to H 20 2 production via auto-oxidation of ferredoxin even in the presence of an oxygen trap (glucose and glucose oxidase). U nder optimum conditions of ferredoxin concentration the leak can be m inimized such th at H 2 production is about 70% of the ferricyanide Hill reaction rate. 
  Reference    Z. Naturforsch. 33c, 113 (1978); received October 5 1977 
  Published    1978 
  Keywords    Biophotolysis, H 2 Production, Hydrogenase 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0113.pdf 
 Identifier    ZNC-1978-33c-0113 
 Volume    33 
2Author    Ian Fry, George Papageorgiou, Elisha Tel-Or, Lester PackerRequires cookie*
 Title    Reconstitution of a System for H 2 Evolution with Chloroplasts, Ferredoxin, and Hydrogenase  
 Abstract    Continuous light-dependent H2 production was studied in a reconstituted in vitro system using S pin acea oleracea chloroplasts, C lostridiu m pasteurianum hydrogenase and S piru lin a m axim a fer­ redoxin. Photosystem Il-dependent production at 30 °C is 60 —70 /^mol H2/mg chlorophyll. At 
  Reference    (Z. Naturforsch. 32c, 110 [1977]; received December 1 1976) 
  Published    1977 
  Keywords    Biophotolysis, H2 Production, Hydrogenase, Ferredoxin, Chloroplasts 
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 TEI-XML for    default:Reihe_C/32/ZNC-1977-32c-0110.pdf 
 Identifier    ZNC-1977-32c-0110 
 Volume    32 
3Author    PatrickC. Hallenbeck, LeonV. Kochian, JohnR. BenemannRequires cookie*
 Title    Hydrogen Evolution Catalyzed by Hydrogenase in Cultures of Cyanobacteria  
 Abstract    Cultures of Anabaena cylindrica, grown on media containing 5 m M N H 4C1 (which represses heterocyst formation), evolved hydrogen after a period o f dark incubation under an argon atmosphere. This hydrogen production was not due to nitrogenase activity, which was nearly undetectable, but was due to a hydrogenase. Cultures grown on media with tungsten substituted for molybdenum had a high frequency of heterocysts (15%) and inactive nitrogenase after nitrogen starvation. The hydrogenase activity of these cultures was three-fold greater than the activity of non-heterocystous cultures. The effects o f oxygen inhibition on hydrogen evolution by hetero-cystous cultures suggest that two pools o f hydrogenase activity exist — an oxygen sensitive hydrogen evolution in vegetative cells and a relatively oxygen-resistent hydrogen evolution in heterocysts. In either case, inhibition by oxygen was reversible. Light had an inhibitory effect on net hydrogen evolution. Hydrogen production in vitro was much higher than in vivo, indicating that in vivo hydrogenase activity is limited by endogenous reductant supply. 
  Reference    Z. Naturforsch. 36c, 87 (1981); received June 13/August 25 1980 
  Published    1981 
  Keywords    Hydrogenase, Cyanobacteria, Heterocysts, Hydrogen Evolution, Hydrogen Metabolism 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0087.pdf 
 Identifier    ZNC-1981-36c-0087 
 Volume    36 
4Author    Thom As Urbig, Rüdiger Schulz, H. Orst SengerRequires cookie*
 Title    Inactivation and Reactivation of the Hydrogenases of the Green Algae Scenedesmus obliquus and Chlamydomonas reinhardtii  
 Abstract    The hydrogenases of the green algae Scenedesmus obliquus and Chlamydomonas reinhardtii were activated under anaerobic conditions. Exposure o f whole cells and cell-free homogenates to air lead to a complete inactivation of the hydrogenases. The inactivation in whole cells of Scenedesmus is faster than the inactivation of the cell-free homogenate. Inactivation o f the hy­ drogenases could be reversed by anaerobic readaptation in whole cells. The inactivation of the hydrogenase in homogenates seems to be irreversible. N either the removal o f oxygen nor the addition of ATP, N AD(P)H, sodium dithionite, dithiothreitol, ferredoxin and thioredoxin to homogenates facilitated the reactivation o f the hydrogenase. The occurrence of a hydrogenase regulating factor is discussed. 
  Reference    Z. Naturforsch. 48c, 41 (1993); received September 30/December 21 1992 
  Published    1993 
  Keywords    Scenedesmus obliquus, Chlamydomonas reinhardtii, Hydrogenase, Reactivation Process, Inactivation 
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 TEI-XML for    default:Reihe_C/48/ZNC-1993-48c-0041.pdf 
 Identifier    ZNC-1993-48c-0041 
 Volume    48 
5Author    J.-H KlemmeRequires cookie*
 Title    Photoproduction of Hydrogen by Purple Bacteria: A Critical Evaluation of the Rate Limiting Enzymatic Steps  
 Abstract    The enzymatic mechanisms and energetics o f nitrogenase-catalyzed photoproduction o f hydrogen from organic C -com pounds by purple bacteria are discussed in respect to the ques­ 
  Reference    Z. Naturforsch. 48c, 482 (1993); received January 15 1993 
  Published    1993 
  Keywords    Hydrogen, Hydrogenase, N itrogenase, Purple Bacteria, Photosynthesis 
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 TEI-XML for    default:Reihe_C/48/ZNC-1993-48c-0482.pdf 
 Identifier    ZNC-1993-48c-0482 
 Volume    48