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'Hydrogenase' in keywords Facet   section ZfN Section C:Volume 036  [X]
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1981 (1)
1Author    PatrickC. Hallenbeck, LeonV. Kochian, JohnR. BenemannRequires cookie*
 Title    Hydrogen Evolution Catalyzed by Hydrogenase in Cultures of Cyanobacteria  
 Abstract    Cultures of Anabaena cylindrica, grown on media containing 5 m M N H 4C1 (which represses heterocyst formation), evolved hydrogen after a period o f dark incubation under an argon atmosphere. This hydrogen production was not due to nitrogenase activity, which was nearly undetectable, but was due to a hydrogenase. Cultures grown on media with tungsten substituted for molybdenum had a high frequency of heterocysts (15%) and inactive nitrogenase after nitrogen starvation. The hydrogenase activity of these cultures was three-fold greater than the activity of non-heterocystous cultures. The effects o f oxygen inhibition on hydrogen evolution by hetero-cystous cultures suggest that two pools o f hydrogenase activity exist — an oxygen sensitive hydrogen evolution in vegetative cells and a relatively oxygen-resistent hydrogen evolution in heterocysts. In either case, inhibition by oxygen was reversible. Light had an inhibitory effect on net hydrogen evolution. Hydrogen production in vitro was much higher than in vivo, indicating that in vivo hydrogenase activity is limited by endogenous reductant supply. 
  Reference    Z. Naturforsch. 36c, 87 (1981); received June 13/August 25 1980 
  Published    1981 
  Keywords    Hydrogenase, Cyanobacteria, Heterocysts, Hydrogen Evolution, Hydrogen Metabolism 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0087.pdf 
 Identifier    ZNC-1981-36c-0087 
 Volume    36