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1Author    MatthewO. Ilori, Dan-Israel AmundRequires cookie*
 Title    Production of a Peptidoglycolipid Bioemulsifier by Pseudomonas aeruginosa Grown on Hydrocarbon  
 Abstract    A strain of Pseudomonas aeruginosa isolated from a polluted soil was found to produce an extracellular bioemulsifier when cultivated on hexadecane as sole carbon source. The emulsifier was precipitated with acetone and redissolved in sterile water. D odecane, crude oil and kerosene were found to be good substrates for emulsification by the bioemulsifier. Growth and bioemulsifier production reached the optimal levels on the fourth and fifth day, respectively. Emulsifying activity was observed over a pH range o f 3.5 to 10.0 with a maxi­ mum at pH 7.0. The activity o f the bioemulsifier was heat stable up to 70 °C while about 50 percent of its activity was retained at 100 °C. The com ponents of the bioem ulsifier were determined, it was found to contain carbohydrate, protein and lipid. The protein complex was precipitated with ammonium sulphate and fractionated on a Sephadex G-100. Gel elec­ trophoresis of the bioemulsifier showed a single band whose molecular weight was estimated as 14,322 Da. The bioemulsifier was classified as a peptidoglycolipid. Certain strains of P. aeruginosa produce peptidoglycolipid in place o f rhamnolipid. 
  Reference    Z. Naturforsch. 56c, 547 (2001); received February 6 /March 8 2001 
  Published    2001 
  Keywords    Pseudom onas, Hydrocarbon, Peptidoglycolipid 
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 TEI-XML for    default:Reihe_C/56/ZNC-2001-56c-0547.pdf 
 Identifier    ZNC-2001-56c-0547 
 Volume    56