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1998 (1)
1Author    BeateM. EierRequires cookie*
 Title    Superoxide Release in Human Fibroblasts upon Treatment with Culture Supernatants of the Arthritogenic Bacteria Erysipelothrix rhusiopathiae and Mycoplasma arthritidis  
 Abstract    Culture supernatants of the arthritogenic bacteria Mycoplasma pneum onia, M ycoplasm a arthritidis, Borrelia burgdorferi and Erysipelothrix rhusiopathiae stimulated primary cultures of human fibroblasts to release reactive oxygen species into the environment, whereas cell walls and membranes of these bacteria had no effects. Lipopolysaccharides of various gram-negative bacteria and lipid A , the lipid moiety of endotoxines, also failed to stimulate the release of reactive oxygen species by fibroblasts. The stimulatory fractions of the culture supernatants of M ycoplasma arthritidis and E rysip­ elothrix rhusiopathiae exhibited a molecular weight of about 9.5 kDa. After an induction period of 5 min the presence o f the stimulant was not necessary any more. The primary radical released by the fibroblasts was the superoxide anion 0 2'. Radical formation took place continuously over som e hours. Additionally, low-level chemiluminescence of fibroblasts was increased upon stimulation with culture supernatants of M ycoplasm a arthritidis and E ry­ sipelothrix rhusiopathiae. N o irreversible injury o f the fibroblast was caused upon stimulation and the cells exhibited normal proliferation pattern after replacing them to the culture medium. pathiae T28 and Mycoplasma arthritidis I SRI, which serve as a model for rheum atoid arthritis in man (Ajmal, 1970; Cole et al., 1972; Drews, 1972; Schulz, 1980; Schulz et al., 1974; Sikes, 1959). E ry­ sipelothrix rhusiopathiae is a small, gram-positive non mobile coccobacillus causing a chronic arthri­ tis in pigs and rats (Ward, 1922), which has an increasing interest due to its similarities to rheu­ matoid arthritis in man. Beside this pathogenicity it induces natural infections in a wide range of ani­ mals and men (Schulz, 1974; Ajmal, 1969). Mycoplasma arthritidis is the causative agent of arthritis in rats and mice and as Erysipelothrix ar­ thritis represent animal models for rheum atoid ar­ thritis in man (Cole et al., 1971). Mycoplasma are small bacteria lacking a cell wall. Some species posses oligosaccharide chains bound to diglyceride (lipoglycan), which are soluble in organic solvents (Seid et al., 1980). Like the lipopolysaccharides (LPS) of gram negative bacteria they have endo­ toxin-like activities (Razin, 1978). The mem branes of Mycoplasma arthritidis are described to lack these lipoglycans (Razin, 1978). It is unknown whether lipoglycans or cell m em branes of Myco-0939-5075/98/0300-0254 $ 06.00 © 1998 Verlag der Zeitschrift für Naturforschung. All rights reserved. 
  Reference    Z. Naturforsch. 53c, 254 (1998); received D ecem ber 19 1997/February 3 1998 
  Published    1998 
  Keywords    Human Fibroblasts, Superoxide Production, Chemiluminescence, Lipopolysaccharide, Arthritogenic Bacteria 
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 TEI-XML for    default:Reihe_C/53/ZNC-1998-53c-0254.pdf 
 Identifier    ZNC-1998-53c-0254 
 Volume    53