1 | Author | W. Schröder, H. Stieve, I. Classen-Linke | Requires cookie* |
Title | Accessibility of Extracellular Space in the Rhabdome of the Living Isolated Retina of the Crayfish ![]() | ||
Abstract | Diffusion of the tracer enzyme horseradish peroxidase (HRP) into the extracellular spaces of living isolated crayfish retinas was monitored by light-and electronmicroscopic identification of the reaction product obtained after immobilisation of the enzyme by fixation. During the incuba tion in enzyme containing saline (up to 3 hours) the extracellularly recorded electroretinogram (ERG) stayed normal and showed no substantial change. The reaction product of the enzyme HRP was found only extracellularly in the space between the microvilli in the rhabdome. The results indicate an exchange of substances via extracellular pathways between the extra cellular side of the photosensory membrane and the bathing fluid in the living isolated crayfish retina. | ||
Reference | Z. Naturforsch. 34c, 136 (1979); received November 7 1978 | ||
Published | 1979 | ||
Keywords | Light Microscopy, Electron Microscopy, Horseradish Peroxidase Tracer Labelling, Electroretinogram, Retina, Extracellular Compartments | ||
Similar Items | Find |