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'Horseradish Peroxidase Tracer Labelling' in keywords
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1979 (1)
1Author    W. Schröder, H. Stieve, I. Classen-LinkeRequires cookie*
 Title    Accessibility of Extracellular Space in the Rhabdome of the Living Isolated Retina of the Crayfish  
 Abstract    Diffusion of the tracer enzyme horseradish peroxidase (HRP) into the extracellular spaces of living isolated crayfish retinas was monitored by light-and electronmicroscopic identification of the reaction product obtained after immobilisation of the enzyme by fixation. During the incuba­ tion in enzyme containing saline (up to 3 hours) the extracellularly recorded electroretinogram (ERG) stayed normal and showed no substantial change. The reaction product of the enzyme HRP was found only extracellularly in the space between the microvilli in the rhabdome. The results indicate an exchange of substances via extracellular pathways between the extra­ cellular side of the photosensory membrane and the bathing fluid in the living isolated crayfish retina. 
  Reference    Z. Naturforsch. 34c, 136 (1979); received November 7 1978 
  Published    1979 
  Keywords    Light Microscopy, Electron Microscopy, Horseradish Peroxidase Tracer Labelling, Electroretinogram, Retina, Extracellular Compartments 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0136.pdf 
 Identifier    ZNC-1979-34c-0136 
 Volume    34