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1Author    Jürgen Bode, Ulridi-Dieter StandtRequires cookie*
 Title    Structural Aspects of Histone Complexes and Nucleosomes Revealed by the Accessibility of Cysteine Side Chains  
 Abstract    Chemical modification with 5,5'-dithiobis-(2-nitrobenzoic acid) shows that histone H3 cysteines 110 (chicken) or 96 and 110 (calf) are com pletely protected in native chromatin and core particles but become unm asked sim ultaneously during a salt induced dissociation. In whole histone extracted from chicken erythrocyte chromatin, H3 Cys-110 residues experience a uniform environ­ m ent at 2 m NaCl and pH 5.5. In whole histone extracted under the same conditions from calf thymus, H3 Cys-96 provides a more accessible thiol group than Cys-110. At higher pH values a conform ational heterogeneity is induced causing a p artial exposure of both cysteine side chains. The kinetic approach described in the present work provides a highly sensitive means to probe the homogeneity of H3 containing protein complexes. 
  Reference    Z. Naturforsch. 33c, 884 (1978); received August 2 1978 
  Published    1978 
  Keywords    Histone, Chrom atin, Protein SH-Reactivity, Nucleosomes 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0884.pdf 
 Identifier    ZNC-1978-33c-0884 
 Volume    33 
2Author    Jan PalygaRequires cookie*
 Title    A Disappearance of a 24-kDa Acid-Soluble Protein from Liver Chromatin of Normal and Starved Hens Following D-Galactosamine Administration  
 Abstract    Normal and starved adult chickens were injected intraperitoneally with D-galactosamine hydro­ chloride (0.5 g/kg body weight) and 6 h later liver chromatin acid-soluble proteins were isolated. These proteins were resolved by a two-dimensional polyacrylamide gel electrophoresis in the presence of non-ionic detergent, Triton X-100, in the first dimension and anionic detergent, sodium dodecyl sulfate, in the second dimension. Although spotting patterns of acid-soluble chromatin proteins were remarkably similar between normal and starved control birds and those receiving D-galactosamine, a disappearance of a 24-kDa protein after administration of this agent was found. Moreover, it was shown that this protein was also completely absent in the chicken erythrocyte chromatin which was known to be inactive in RNA synthesis. It seems that the disappearance of the 24-kDa chromatin protein may be associated with inhibiting of transcription in hen liver after D-galactosamine administration and during hen erythrocyte maturation. 
  Reference    Z. Naturforsch. 40c, 798—805 (1985); received August 13 1985 
  Published    1985 
  Keywords    D-Galactosamine, Starvation, Chicken Liver, Chromatin, Histones 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0798.pdf 
 Identifier    ZNC-1985-40c-0798 
 Volume    40 
3Author    IgorZ. Zubrzycki3, Lothar BohmbRequires cookie*
 Title    Folding of the SPKK Rich Peptide in the Presence of the Octa-Oligonucleotide  
 Abstract    The nucleosom e contains o f 200 base pairs of D N A com plexed with four core histone complex: H 2A , H2B, H3, and H4. The fifth histone species, the HI histone, interacts with linker D N A connecting neighbouring nucleosomes. We have studied the influence o f the phosphorylation on the interactions of a repeating unit 15 residues long, containing the SPKK motif, the m otif thought to induce turn along peptides sequences, enclosed within the trout testis H I C-terminal domain with octanucleotide by means of the thermal denaturation and CD technique. The results indicate that the peptide preferentially binds to a single stranded oligonucleotide. It has been shown further that there is no ß structure present but a distorted helical structure has been detected. 
  Reference    Z. Naturforsch. 52c, 77—8 (1997); received March 11/August 19 1996 
  Published    1997 
  Keywords    N ucleosom e, Histone, Circular Dichroism, Thermal Denaturation 
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 TEI-XML for    default:Reihe_C/52/ZNC-1997-52c-0077.pdf 
 Identifier    ZNC-1997-52c-0077 
 Volume    52 
4Author    W. Pyerin, N. Baibach, D. Kübler, V. KinzelRequires cookie*
 Title    Protein Kinases in HeLa Cells and in Human Cervix Carcinoma  
 Abstract    Extracts of HeLa cell fractions were analyzed by DEAE-and phospho-cellulose chromatog­ raphy for their range o f cyclic AMP-dependent and -independent protein kinase activities phosphorylating histone and/or phosvitin; extractions were by phosphate buffered saline (soluble protein kinases) and the non-ionic detergent NP-40 (membrane-bound protein kinases). The soluble fraction contained (i) cyclic AMP-dependent histone kinases type I and II as evidenced by their behaviour on DEAE-cellulose and inhibition by the specific heat-and acid-stable protein kinase inhibitor (PKI) in a dose-related manner; both types I and II as well as their purified catalytic subunit also phosphorylated protamine and — with very low efficiency -casein but not phosvitin; (ii) a histone kinase (H), insensitive to cyclic AMP and PKI, also accepting protamine as substrate but not either casein or phosvitin; (iii) a phosvitin kinase (P), insensitive to cyclic AMP and PKI, which also phosphorylates casein but not histone or protamine. These four enzyme species were also found in NP-40 extracts o f 2 7 0 0 0x g residues which, however, contained further histone and phosvitin kinase activities as yet unspecified. NP-40 extracts o f the microsomal fraction possessed, besides unspecified histone and phosvitin kinase activity, only the phosvitin kinase P and appeared to be devoid of histone kinases I, II, and H. The occurrence and ratios o f the protein kinases classified suggest an ordered distribution over the diverse subcellular fractions of HeLa cells. The overall pattern of soluble and membrane-bound histone and phosvitin kinases in extracts of cervix carcinoma tissue, the in vivo correlate o f HeLa cells, closely resembled that of similar extracts of HeLa cells. HeLa cells hence appear, despite their long in vitro history, to express protein kinase activities similar to those o f their in vivo ancestors, recommending them as a subject for the study of (certain) human protein kinase systems. 
  Reference    Z. Naturforsch. 36c, 552—561 (1981); received December 4 1980/February 161981 
  Published    1981 
  Keywords    Protein Kinases, Cyclic AMP-Dependent and -Independent, HeLa Cells, Human Cervix Carcinoma, Histone, Phosvitin 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0552.pdf 
 Identifier    ZNC-1981-36c-0552 
 Volume    36