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'Histidine Modification' in keywords Facet   Publication Year 1989  [X]
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1989[X]
1Author    JaspreetA. Rora, P. V. SaneRequires cookie*
 Title    Histidyl Residues Mediate Electron Transport in Plant Mitochondria  
 Abstract    Plant The effect of chemical modification of histidyl residues using diethyl pyrocarbonate (D EP) on plant mitochondrial electron transport was studied. Mitochondrial membranes from potato tubers were isolated and electron flow from N A D H to oxygen, NADH to ferricyanide and ascorbate to oxygen were monitored in pro», nee and/or absence of DEP. Measurements were made at various concentrations of DEP and at different pHs either by using an oxygen electrode or spectro-photometrically. The results show that DEP inhibits flow of electrons from NADH to oxygen, however partial electron transport from N A D H to ferricyanide and ascorbate to oxygen was unaffected. Maximum inhibition was observed at pH 6.5. The time course of the DEP action revealed a biphasic nature of inhibition. Effects on the levels of reduction of cytochromes b and c by DEP during electron transport indicated that histidyl residues may be present before or at cytochrome b , which are being modified. 
  Reference    Z. Naturforsch. 44c, 537 (1989); received April 7 1989 D edicated to 
  Published    1989 
  Keywords    Mitochondria, Histidine Modification, Electron Transport, Spectral Studies 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0537.pdf 
 Identifier    ZNC-1989-44c-0537 
 Volume    44