| | 2 | Author
| Naaman Akaviaa, Dieter Strackb, Avner Cohen3 | Requires cookie* | | | Title
| The Coloration of Gladiolus I. Survey of Anthocyanins in Petals of Gladiolus  | | | | Abstract
| The six common anthocyanidins found in Gladiolus petals occur in four types o f glycosilation: 3-glucoside, 3-rhamnoglucoside, 3,5-diglucoside, and 3-rhamnoglucoside-5-glucoside. The six monoglucosides appear in minute quantities, whereas any of the other 18 anthocyanins can serve as the m ajor contributor to the coloration of Gladiolus petals. In high performance liquid chrom atographic analyses of petal pigm ent composition o f nine cultivars, it was found that the anthocyanins are grouped on the basis of the aglycon substitution. Thus, pelargonidin appears by itself (group I), cyanidin and peonidin constitute group II, and delphinidin, petunidin, and malvidin group III. | | | |
Reference
| Z. Naturforsch. 36c, 378 (1981); received January 28 1981 | | | |
Published
| 1981 | | | |
Keywords
| Anthocyanins, High Performance Liquid Chromatography, Gladiolus, Iridaceae | | | |
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| | | | | TEI-XML for
| default:Reihe_C/36/ZNC-1981-36c-0378.pdf | | | | Identifier
| ZNC-1981-36c-0378 | | | | Volume
| 36 | |
| 3 | Author
| Wolfgang Knogge, Gottfried Weissenböck, Dieter Strack | Requires cookie* | | | Title
| Application of Liquid Chromatography to a Study on 4-Coumarate: Coenzyme A Ligase Activity | | | | Abstract
| This report describes the separation of components from a 4-coumarate: CoA ligase assay by means of liquid chromatography. With the aid o f polyamide column chromatography it is possible to enrich and isolate chromatographically and UV spectroscopically pure /»-coumaroyl-CoA using as a solvent 0.01% N H 4OH in methanol subsequent to water and methanol alone. High performance liquid chromatography on octadecylsilane-bonded silica stationary phase allows a discontinuous determination o f ligase activity. All components — ATP, Coenzyme A, p-coumaric acid, and the products AMP and p-coumaroyl-CoA — can be separated and accurately quantified within 20 min using a water-acetonitrile gradient, containing 1% phosphoric acid. The presented HPLC method may be used to affirm the accuracy of optical tests. | | | |
Reference
| Z. Naturforsch. 36c, 197—199 (1981); received December 91980 | | | |
Published
| 1981 | | | |
Keywords
| Avena sativa, Poaceae, Ligase, /?-Coumaroyl-CoA, Polyamide Column Chromatography, High Performance Liquid Chromatography | | | |
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| default:Reihe_C/36/ZNC-1981-36c-0197.pdf | | | | Identifier
| ZNC-1981-36c-0197 | | | | Volume
| 36 | |
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