| | 1 | Author
| D. Ieter, S. Track3, N. Aam, A. Kaviab, HansR. Eznika | Requires cookie* | | | Title
| High Performance Liquid Chromatographic Identification of Anthocyanins  | | | | Abstract
| A high performance liquid chromatographic procedure for a rapid identification o f antho cyanins is described. Besides their characteristic retention times, anthocyanins can easily be identified by an HPLC examination o f the kinetics of appearance and disappearance o f their partial hydrolysis products. This method is also applicable to 3-deoxyanthocyanins which is demonstrated with an extract from the fern Blechnum brasiliense. The described method allows optimal resolution of complex anthocyanin mixtures in the fol lowing sequence o f elution: 3-sophoroside-5-glucoside; 3-rutinoside-5-glucoside; 3,5-diglucoside; 3-sophoroside; 3-galactoside; 3-glucoside; 3-rutinoside; 5-glucoside; aglycone, and finally the forms acylated with /?-coumaric acid. Substitution in the B-ring o f the aglycone structure is the key factor for the range o f the retention time. Thus cyanidin 3,5-diglucoside elutes earlier (—38 sec) than pelargonidin 3-sophoroside-5-glucoside. | | | |
Reference
| Z. Naturforsch. 35c, 533—538 (1980); received March 20 1980 | | | |
Published
| 1980 | | | |
Keywords
| Anthocyanins, 3-Deoxyanthocyanins, Partial Hydrolysis, High Performance Liquid Chromato graphy | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/35/ZNC-1980-35c-0533.pdf | | | | Identifier
| ZNC-1980-35c-0533 | | | | Volume
| 35 | |
|
