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'Herbicide Resistance' in keywords Facet   Publication Year 1995  [X]
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1995[X]
1Author    Jack Farineaua, DanielleM. Laval-, ArtinbRequires cookie*
 Title    Characteristics of Thermoluminescence Bands of Euglena Cells Belonging to 2 Lines Presenting Different Degrees of Diuron-Resistance  
 Abstract    We have analysed the thermoluminescence (TL) properties of two lines o f Euglena exhibit­ ing two degrees of resistance to diuron, by a factor of 100 (Z R 25) and 1000 (Z R 250) respectively, as compared to wild type line (Z). In addition, the two ZR lines developped an identical resistance to atrazine since the I50 for this herbicide in each line was 75 times larger than in wild type. Special TL characteristics were evidenced in the two lines. Bands after 2 flashes (or more) showed a shift o f the peak maximum towards low temperature, the shift being the largest in the most DCM U-resistant cells. Similar results were obtained with isolated thylakoids, except that the TL bands appeared at a temperature higher than in corresponding cells. Oscillations in the amplitude of the bands in a flash sequence were largely damped in cells (and thylakoids), particularly in the most DCM U-resistant lines. The results are interpreted as indicating accumulation of Q a "Qb after flashes due to a decrease of the equilibrium constant for the reaction Q a ~Qb ^ QaQb~ accompanying the D CM U resistance. 
  Reference    Z. Naturforsch. 50c, 86—9 (1995); received October 12/November 21 1994 
  Published    1995 
  Keywords    Diuron, Euglena, Herbicide-Resistance, Photosystem II, Thermoluminescence 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0086.pdf 
 Identifier    ZNC-1995-50c-0086 
 Volume    50 
2Author    Josef Vinnemeier, Wolfgang Dröge-Laser, ElfriedeK. Pistorius, Inge BroerRequires cookie*
 Title    Purification and Partial Characterization of the Streptomyces viridochromogenes TÜ494 Phosphinothricin-N-acetyltransferase Mediating Resistance to the Herbicide Phosphinothricin in Transgenic Plants  
 Abstract    A purification scheme for the enzyme phosphinothricin-N-acetyltransferase (PAT) origi­ nating from Streptomyces viridochrom ogenes {pat-gene product from Streptom yces virido­ chromogenes) and mediating herbicide resistance to transgenic plants was developed. The enzyme was isolated from a transformed and overproducing Escherichia coli strain. With a combination of ammonium sulfate fractionation, chromatography on D EAE-Sephadex A50-, Phenylsepharose-, Hydroxylapatite-and FPLC-Superose 12-columns it was possible to obtain PAT which was at least 90 % homogeneous on the basis of SDS-PAGE. The properties of the isolated PAT were compared with the properties of PAT from S. hygroscopicus (bar-gene product from S. hygroscopicus) previously isolated and characterisized by Botterman, J., Gossele, V., Thoen, C., Lauwereys, M. (1991), G ene 102, 33-37. Differences were observed in the molecular masses of the two native enzymes (PAT from S. viridochrom ogenes being a dimer of 40 kD and PAT from S. hygroscopicus being a m onomer of 21 kD), and in the tem perature sensitivity of the two enzymes (the PAT from S. viridochrom ogenes being slightly more tem perature stable than PAT from S. hygroscopicus). However, since the pat and the bar-gene are to 85 % homologous, substantial similarities exist between the two enzymes especially in the kinetic values and the substrate specificity. The isolated S. virido­ chromogenes PAT did not acetylate putative substrates present in the plant cell. Antibodies were raised against the isolated protein. This antiserum was able to detect PAT in transgenic plants and therefore is suitable to analyse the fate of the protein in such plants under various stress conditions. 
  Reference    Z. Naturforsch. 50c, 796—805 (1995); received August 16 1995 
  Published    1995 
  Keywords    Biosafety, Herbicide Resistance, Nicotiana tabacum, Phosphinothricin-A'-acetyltransferase (PAT), Transgenic Plants 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0796.pdf 
 Identifier    ZNC-1995-50c-0796 
 Volume    50