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'Herbicide Binding' in keywords Facet   Publication Year 1981  [X]
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1Author    Henrik Laasch, Klaus Pfister, Wolfgang UrbachRequires cookie*
 Title    Comparative Binding of Photosystem II — Herbicides to Isolated Thylakoid Membranes and Intact Green Algae  
 Abstract    The binding of the photosystem II herbicides diuron (DCM U), atrazine (s-triazine), ioxynil and dinoseb (substituted phenols) to isolated spinach thylakoids was saturated in less than 2 min in the dark. In intact cells of the green alga Ankistrodesmus b. it took 10 to 20 min to reach the binding equilibrium. Binding affinity of diuron, atrazine, dinoseb, measured as equilibrium binding constants, was found to be comparable in isolated thylakoids and intact algal cells. For ioxynil, reduced binding affinity was observed in algae. The concentration of binding sites in thylakoids and intact cells was determined to be 300-500 chl/inhibitor binding site, suggesting a 1:1 stoichiometry between bound herbicide and electron transport chains. In intact cells only the phenol herbicides ioxynil and dinoseb showed increased concentrations of binding sites. Strong correlation of herbicide binding and inhibition o f electron transport was found for diuron in isolated thylakoids and intact cells. In thylakoids this is valid also for atrazine and dinoseb. For ioxynil a difference between the amount of binding and inhibition was found. This correlation o f herbicide binding and inhibition proves that binding specifically occurs at the inhibition site at photosystem H. In addition to the specific binding, for all four herbicides studied, (except for ioxynil in thylakoids) unspecific binding was observed in thylakoids as well as in algae, which was not related to inhibition. 
  Reference    Z. Naturforsch. 36c, 1041—1049 (1981); received July 13 1981 
  Published    1981 
  Keywords    Herbicides, Herbicide Binding, Photosynthesis, Thylakoids, Algae 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-1041.pdf 
 Identifier    ZNC-1981-36c-1041 
 Volume    36