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'Hemolysis' in keywords Facet   section ZfN Section C  [X]
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1Author    Dieter Auer, Gerhard BrandnerRequires cookie*
 Title    Loading of H um an Red Blood Cells with D N A and R N A  
 Abstract    Human erythrocytes were suspended in Hank's solution containing mammalian or viral D N A or R N A . After dialysis at 0 ° C first against water and subsequently against Hank's solution, and a further incubation at 37 °C , the erythrocytes were found to be loaded with the nucleic acids. The nucleic acid trapped in the erythrocytes exhibited up to 35 per cent of the external con­ centration. 
  Reference    (Z. Naturforsch. 31c, 149 [1976]; received November 24 1975) 
  Published    1976 
  Keywords    Erythrocytes, Trapping, Gene Transfer, Hemolysis, SV40 D N A 
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 TEI-XML for    default:Reihe_C/31/ZNC-1976-31c-0149.pdf 
 Identifier    ZNC-1976-31c-0149 
 Volume    31 
2Author    Daniela Vedaldi, Sergio Caffieri, Giorgia Miolo, Francesco Dall 'acqua, Paola ArslanRequires cookie*
 Title    Dark and Photohemolysis of Erythrocytes by Furocoumarins  
 Abstract    It has been shown that various furocoumarins are able to cause dark hemolysis in red blood cells (RBC). However, this effect is evident only at relatively high furocoumarin concentrations (4.6 x 10~ 4 M) -much higher than those used in photosensitization experiments or photo-chemotherapeutic treatments. Among the various furocoumarins examined in this study, only psoralen (Ps) and 3-carbethoxy-psoralen (3-CPs) showed strong photohemolytic effects, while the other compounds revealed little or no activity. This fact indicates that Ps and 3-CPs are able to induce selective damage to the cell membrane of RBC. By pre-irradiating furocoumarin in ethanol or isotonic saline solutions and adding the irradiated solutions to a RBC suspension, hemolysis was observed in various compounds. The products of photolysis which form during pre-irradiation may be responsible, in terms of hemolysis, for toxic effects on RBC. 
  Reference    Z. Naturforsch. 43c, 888—892 (1988); received August 2 1988 
  Published    1988 
  Keywords    Furocoumarins, Hemolysis, Photosensitization, Cell Membrane Damage, Photochemotherapy 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0888.pdf 
 Identifier    ZNC-1988-43c-0888 
 Volume    43 
3Author    H. Kleszczyńska, J. Hładyszowski, H. Pruchnik, S. PrzestalskiRequires cookie*
 Title    Erythrocyte Hemolysis by Organic Tin and Lead Compounds  
 Abstract    The effect of trialkyllead and trialkyltin on pig erythrocyte hemolysis has been studied and compared. The results o f experiments showed that the hemolytic activity o f organoleads increases with their hydrophobicity and follows the sequence: triethyllead chloride < tri-n-propyllead chloride < tributyllead chloride. And similarly in the case of organotins: triethyltin chloride < tri-n-propyltin chloride < tributyltin chloride. Comparison of the hemolytic activity of organoleads and organotins indicates that the lead compounds exhibit higher hemolytic activity. The methods of quantum chemistry allowed to determine the maximum electric potential of the ions R3Pb+ and R3Sn+, and suggest a relationship betw een the potential and toxicity. 
  Reference    Z. Naturforsch. 52c, 65—6 (1997); received August 9/October 7 1996 
  Published    1997 
  Keywords    Organoleads, Organotins, Erythrocyte Membrane, Hemolysis, Electric Potential 
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 TEI-XML for    default:Reihe_C/52/ZNC-1997-52c-0065.pdf 
 Identifier    ZNC-1997-52c-0065 
 Volume    52 
4Author    Janusz Sarapuk3, Halina Kleszczyriska3, JuliuszJoanna Pernakb, Bozenna Kalewskab, Rözycka-Roszak3Requires cookie*
 Title    Influence of Counterions on the Interaction of Pyridinium Salts with Model Membranes  
 Abstract    The interaction of pyridinium salts (PS) with red blood cells and planar lipid membranes was studied. The aim of the work was to find whether certain cationic surfactant counterion influence its possible biological activity. The counterions studied were C L , Br", I-, C 104~, BF4~ and N 0 3_. The model membranes used were erythrocyte and planar lipid membranes (BLM). At high concentration the salts caused 100% erythrocyte hemolysis (C 10o) or broke BLMs (CC). Both parameters describe mechanical properties of model membranes. It was found that the efficiency of the surfactant to destabilize model membranes depended to som e degree on its counterion. In both, erythrocyte and BLM experiments, the highest efficiency was observed for Br_, the lowest for N 0 3_. The influence o f all other anions on surfactant efficiency changed between these two extremities; that of chloride and perchlorate ions was similar. Some differences were found in the case o f BF4~ ion. Its influence on hemolytic possibilities of PS was significant while BLM destruction required relatively high concentration of this anion. Apparently, the influence of various anions on the destructive action of PS on the model membrane used may be attributed to different mobilities and radii o f hydrated ions and hence, to different possibilities o f particular anions to modify the surface potential of model membranes. This can lead to a differentiated interaction o f PS with m odi­ fied bilayers. Moreover, the effect of anions on the water structure must be taken into ac­ count. It is important whether the anions can be classified as water ordering kosm otropes that hold the first hydration shell tightly or water disordering chaotropes that hold water m olecules in that shell loosely. 
  Reference    Z. Naturforsch. 54c, 952 (1999); received April 6/May 20 1999 
  Published    1999 
  Keywords    M odel Membranes, Hemolysis, Stability, Cationic Surfactants, Counterions 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-0952.pdf 
 Identifier    ZNC-1999-54c-0952 
 Volume    54