| | 1 | Author
| R. Om, G., EligiuszS. Erafin, JanuszM. Azur | Requires cookie* | | | Title
| The Kinetics of the Reaction of Thiocyanate and Nitrite Ions with Orconectes limosus Oxyhemocyanin  | | | | Abstract
| The time course of the reaction of oxyhemocyanin from the arthropod Orconectes limosus with SCN" and N 0 2 " was investigated. After addition of one of these anions to oxyhemocyanin solu tion an exponential decay of A 340 was found, indicating a removal of oxygen from the active site. The order of reaction with respect to ligand was about 3 for thiocyanate and about 1 for nitrite. On this basis, a possible arrangement of ligands in the active site has been proposed. The final product of reaction with SCN" yielded a mononuclear copper EPR spectrum while the reaction product with N 07 gave a characteristic spectrum of two coupled Cu2+ ions. It was possible to remove the ligands from the active site by dialysis. The reaction patterns showed distinct changes in a narrow pH range, 5.3—6.5. A possible effect of structural alterations of the protein moiety on the course of the reaction of oxyhemocyanin with ligands is suggested. | | | |
Reference
| Z. Naturforsch. 40c, 677—681 (1985); received February 4 1985 | | | |
Published
| 1985 | | | |
Keywords
| Hemocyanin, Reaction Kinetics, Copper, Electron Paramagnetic Resonance | | | |
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| default:Reihe_C/40/ZNC-1985-40c-0677.pdf | | | | Identifier
| ZNC-1985-40c-0677 | | | | Volume
| 40 | |
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