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'Hemocyanin Channel' in keywords
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1985 (1)
1984 (1)
1Author    Flavia Pasquali, G. Ianfranco, M. Enestrina, R. Enzo, A. NtoliniRequires cookie*
 Title    Fusion of Large Unilamellar Liposomes Containing Hemocyanin with Planar Bilayer Membranes  
 Abstract    Large unilamellar vesicles were prepared by detergent rem oval from micelles containing phosphatidylcholine, phosphatidylethanolam ine and phosphatidylserine. Liposomes were then interacted with Megathura crenulata hemocyanin, a well studied channel former. Incubation o f the resulting proteoliposomes on one side of a phosphatidylserine-containing planar bilayer under fusion conditions yielded strong current increases. Such increase is due to insertion o f ionic channels from the liposomes into the planar bilayer. Studying the effects o f Ba2+ on the electrical properties of the channel we could show that the protein is always inserted into a bilayer during this process, i.e. fusion of proteoliposomes with the artificial m em brane occurs. The strong non linearity of the current-voltage curve of the hemocyanin pore could be used as a probe o f the extent to which fusion preserves the orientation of the channel through the bilayer. 
  Reference    Z. Naturforsch. 39c, 147—155 (1984); received September 22 1983 
  Published    1984 
  Keywords    Membrane Fusion, Large Liposomes, Planar Bilayer, Hemocyanin Channel, Asymmetrical Insertion 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0147.pdf 
 Identifier    ZNC-1984-39c-0147 
 Volume    39 
2Author    Gianfranco Menestrina, Flavia PasqualiRequires cookie*
 Title    Effects of Carbohydrates on the Ion Conductance of the Hemocyanin Channel  
 Abstract    The effects o f glucose and sucrose on the ionic conductance properties o f the channel formed by Megathura crenulata hemocyanin in planar lipid bilayers have been studied using m em branes o f different compositions. It was found that glucose at high concentrations strongly affects the time constants o f the current relaxations observed in m em branes containing many channels after a step in the voltage clamp from ground to a positive value. At m uch lower concentrations both sucrose and glucose strengthened the binding o f Ba2+ to the protein, what in turn has the effect to shift the conductance voltage curve o f the pore towards negative potentials. The possible mechanism underlying these effects and the analogies with other studies on the interaction o f sugars and alcohols with proteins have been discussed. 
  Reference    Z. Naturforsch. 40c, 85—9 (1985); received October 25 1984 
  Published    1985 
  Keywords    Hemocyanin Channel, Planar Lipid Bilayer, Carbohydrates, D ivalen t Cations, Protein Conformations 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0085.pdf 
 Identifier    ZNC-1985-40c-0085 
 Volume    40