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2001 (1)
1981 (1)
1Author    Ei-Ichiro Fukusaki3, Takashi Oishi3, Hozumi Tanakab, Shin-Ichiro Kajiyama3, Akio Kobayashi3Requires cookie*
 Title    Identification of Genes Induced by Taxol Application Using a Combination of Differential Display RT-PCR and DNA Microarray Analysis  
 Abstract    The differential display reverse transcriptional polymerase chain reaction (DD-RT-PCR) was used to hunt for cDNA fragments specifically expressed by taxol treatment of HeLa cells. Forty-eight cDNA clones were differentially displayed through the experiments. The cDNA fragments obtained were separately spotted onto glass slides to prepare a tailor-made DNA chip. The gene expression pattern of differentially displayed cDNA fragments were checked by DNA microarray analysis. 
  Reference    Z. Naturforsch. 56c, 814—819 (2001); received March 26/May 22 2001 
  Published    2001 
  Keywords    Taxol, HeLa Cells, DNA Microarray 
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 TEI-XML for    default:Reihe_C/56/ZNC-2001-56c-0814.pdf 
 Identifier    ZNC-2001-56c-0814 
 Volume    56 
2Author    W. Pyerin, N. Baibach, D. Kübler, V. KinzelRequires cookie*
 Title    Protein Kinases in HeLa Cells and in Human Cervix Carcinoma  
 Abstract    Extracts of HeLa cell fractions were analyzed by DEAE-and phospho-cellulose chromatog­ raphy for their range o f cyclic AMP-dependent and -independent protein kinase activities phosphorylating histone and/or phosvitin; extractions were by phosphate buffered saline (soluble protein kinases) and the non-ionic detergent NP-40 (membrane-bound protein kinases). The soluble fraction contained (i) cyclic AMP-dependent histone kinases type I and II as evidenced by their behaviour on DEAE-cellulose and inhibition by the specific heat-and acid-stable protein kinase inhibitor (PKI) in a dose-related manner; both types I and II as well as their purified catalytic subunit also phosphorylated protamine and — with very low efficiency -casein but not phosvitin; (ii) a histone kinase (H), insensitive to cyclic AMP and PKI, also accepting protamine as substrate but not either casein or phosvitin; (iii) a phosvitin kinase (P), insensitive to cyclic AMP and PKI, which also phosphorylates casein but not histone or protamine. These four enzyme species were also found in NP-40 extracts o f 2 7 0 0 0x g residues which, however, contained further histone and phosvitin kinase activities as yet unspecified. NP-40 extracts o f the microsomal fraction possessed, besides unspecified histone and phosvitin kinase activity, only the phosvitin kinase P and appeared to be devoid of histone kinases I, II, and H. The occurrence and ratios o f the protein kinases classified suggest an ordered distribution over the diverse subcellular fractions of HeLa cells. The overall pattern of soluble and membrane-bound histone and phosvitin kinases in extracts of cervix carcinoma tissue, the in vivo correlate o f HeLa cells, closely resembled that of similar extracts of HeLa cells. HeLa cells hence appear, despite their long in vitro history, to express protein kinase activities similar to those o f their in vivo ancestors, recommending them as a subject for the study of (certain) human protein kinase systems. 
  Reference    Z. Naturforsch. 36c, 552—561 (1981); received December 4 1980/February 161981 
  Published    1981 
  Keywords    Protein Kinases, Cyclic AMP-Dependent and -Independent, HeLa Cells, Human Cervix Carcinoma, Histone, Phosvitin 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0552.pdf 
 Identifier    ZNC-1981-36c-0552 
 Volume    36