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'Haloferax volcanii' in keywords Facet   Publication Year 1995  [X]
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1Author    Susanne Bickel-Sandkötter, Martina UferRequires cookie*
 Title    Properties of a Dissimilatory Nitrate Reductase from the Halophilic Archaeon Haloferax volcanii  
 Abstract    Grown under anaerobic conditions in presence of nitrate, Haloferax volcanii shows nitrate reduction and accumulation of nitrite in the culture medium. We found a membrane-asso-ciated nitrate reductase, which could easily be solubilized by gently stirring of isolated mem­ branes. Surprisingly, this nitrate reductase requires no NaCl for its activity. A medium pH of 7.5 and high temperatures up to 80 °C are necessary for optimum activity. Kinetic studies showed that the apparent K M was 0.36 mmol/1 for nitrate and 80 ^mol/1 for dithionite-reduced methyl viologen. The respiratory chain inhibitor cyanide effects nitrate reduction noncompe-titively with respect to nitrate with a K \ of 0.3 mmol/1. Azide was a strong inhibitor: The concentration required for half maximal inhibition was 60 |amol/l, whereas thiocyanate and chlorate were much weaker inhibitors. The isolated enzyme was partially purified by frac­ tionated precipitation using polyethylene glycol. SDS gel electrophoresis resulted in three putative subunits of the nitrate reductase of molecular masses of about 100, 61, and 31 kDa. 
  Reference    Z. Naturforsch. 50c, 365—372 (1995); received February 6/February 27 1995 
  Published    1995 
  Keywords    Archaeon, Haloferax volcanii, Dissimilatory Nitrate Reductase, Nitrate Reduction, Inhibition 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0365.pdf 
 Identifier    ZNC-1995-50c-0365 
 Volume    50