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'Hairy R oot Cultures' in keywords
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1991 (2)
1Author    J. Berlinab, C. Rügenhagena, M. Rippert, S. ErdoganRequires cookie*
 Title    Effects of Culture Conditions on Isoflavonoid Levels of Transformed and Non-Transformed Cultures of Lupinus -a Comparison of Suspension and Hairy Root Cultures  
 Abstract    Some highly productive suspension and hairy root cultures were found among several trans­ formed cultures o f Lupinus polyphyllus and L. hartwegii. A transformed suspension culture Lupo 30150 and a root culture Luha 15834 containing the highest specific isoflavone glucoside content were characterized and compared with normal phytohormone-dependent lines with respect to product stability as well as to their responsiveness to external triggers, e.g. response to changes in the medium. While phytohorm one-dependent suspension cultures lost their ini­ tial ability to form increased levels o f isoflavonoids on phytohormone-free medium, the trans­ formed phytohorm one-independent suspension Lupo 30150 remained a highly productive line, despite the fact that its specific levels decreased to 60% o f the initial values during several years in liquid medium. Highest stability o f product patterns and levels were noted for the transformed root culture. Phytohorm ones had little effect on growth and isoflavonoid levels in suspension cultures, while they reduced both strongly in root cultures. In the presence o f 2,4-D the root culture changed into an aggregated low producing suspension culture from which the root state was recovered on phytohorm one-free medium. As long as the root state was main­ tained, isoflavonoid levels could not be distinctly improved by media variation while specific isoflavonoid levels o f suspensions were increased by stress factors such as phosphate depletion. When suspensions were transferred to fresh medium phenylalanine ammonia-lyase was greatly induced within 24 h, while the activity remained nearly unchanged in root cultures. 
  Reference    Z. Naturforsch. 46c, 735—7 (1991); received April 23 1991 
  Published    1991 
  Keywords    Lupinuspolyphyllus, Lupinus hartwegii, Transformation, Hairy R oot Cultures, Isoflavonoids 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0735.pdf 
 Identifier    ZNC-1991-46c-0735 
 Volume    46 
2Author    J. Berlinab, L. Fecker, C. R. Ügenhagenab, C. S. Atorc, D. S. Trackd, L. W. Itted, V. W. RaybRequires cookie*
 Title    Isoflavone Glycoside Formation in Transformed and Non-Transformed Suspension and Hairy Root Cultures of Lupinus polyphyllus and Lupinus hartwegii  
 Abstract    sides Transformed cell suspension and hairy root cultures were established by infecting seedlings o f Lupinus polyphyllus and L. hartwegii with various wild type strains o f Agrobacterium tume-faciens and A. rhizogenes. Transformation o f the cultures was confirmed either by their phyto­ hormone autotrophy, detection o f opines or southern analysis. Glueosides o f genistein and 2'-hydroxygenistein, were found to be the main secondary metabolites in normal and trans­ formed suspension cultures as well as in hairy root cultures. Although some o f the isoflavone glycosides o f the cultures were apparently new constituents o f Lupinus, they were afterwards also found in young seedlings. 
  Reference    Z. Naturforsch. 46c, 725 (1991); received April 23 1991 
  Published    1991 
  Keywords    Lupinus polyphyllus, Lupinus hartwegii, Cell Cultures, Hairy R oot Cultures, Isoflavone Gluco- 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0725.pdf 
 Identifier    ZNC-1991-46c-0725 
 Volume    46