| 1 | Author | J.M F G Aerts, A. W. Schram | Requires cookie* |
| Title | Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals ü . Vacuolar Localization of Some Hydrolases  | ||
| Abstract | In vacuoles isolated from petal upper epiderm is o f Petunia hybrida through polybase-induced lysis o f protoplasts the vacuolar localization o f x-m annosidase and x-galactosidase was dem on strated. N o association was observed between the tonoplast and these hydrolases. Protoplasts from different mutant lines blocked in anthocyanin biosynthesis were treated with DEAE-dextran to release vacuoles. The differences in quality o f the obtained vacuolar preparations indicated the relation between mutant line used and successful application o f the polybase-procedure. | ||
| Reference | Z. Naturforsch. 40c, 196 (1985); received June 26/O ctober 26 1984 | ||
| Published | 1985 | ||
| Keywords | Petunia hybrida, Vacuoles, Anthocyanins, Flavonoids, H ydrolases | ||
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