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1984 (1)
1979 (1)
1Author    N. Pucheu, W. Oettmeier, U. H. Eisterkam, K. Masson, G.F W IldnerRequires cookie*
 Title    Metribuzin-Resistant Mutants of Chlamydomonas reinhardii  
 Abstract    Herbicide resistance in Chlamydomonas reinhardii cells was induced by m utagenesis with 5-fluorodeoxyuridine and ethylm ethanesulfonate. Four m utant strains were isolated and analyzed for resistance against D C M U -type or phenolic inhibitors o f photosynthetic electron transport. The mutants were different in both the extent and the pattern o f their resistance: the R / S value, i.e. the ratio o f / 50 values o f the inhibition o f photosynthetic electron transport in isolated resistant and susceptible thylakoids, varied for m etribuzin from 10 000 to 36. The mutant MZ-1 was resistant against metribuzin, atrazine and D C M U , whereas the mutant M Z-2 showed resistance mainly against metribuzin and atrazine. The m utant M Z-3 was sim ilar to MZ-1, but showed a lesser extent o f resistance against D C M U . The m utant M Z-4 show ed resistance against metribuzin, but not against atrazine. These results dem onstrate that the resistance against one herbicide o f the D C M U -type (m etribuzin) must not be accom panied by sim ilar resistance against te other inhibitors. Binding studies with radioactively labeled herbicides, [l4C]metribuzin, [HC]atrazine and [3H]DCM U, and isolated thylakoids supported these observations. Phosphorylation o f thylakoid m embrane proteins was studied with w ild-type cells and resistant mutants under in vivo conditions in the light. The 32P-labeled m ain proteins bands were in the molecular weight range o f 1 0 -1 4 kDa, 2 6 -2 9 kD a, 3 2 -3 5 kD a and 4 6 -4 8 kDa. The pattern and the extent o f incorporation o f 32P were sim ilar for the m utants and the w ild-type cells. 
  Reference    Z. Naturforsch. 39c, 437—439 (1984); received N ovem ber 30 1983 
  Published    1984 
  Keywords    Metribuzin, Chlamydomonas reinhardii, Q B-Protein, H erbicide R esistance, Thylakoid Phosphorylation 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0437.pdf 
 Identifier    ZNC-1984-39c-0437 
 Volume    39 
2Author    Jonathan GresselRequires cookie*
 Title    A Review of the Place of in vitro Cell Culture Systems in Studies of Action, Metabolism and Resistance of Biocides Affecting Photosynthesis  
 Abstract    The hom ogeneity, density and axenic nature of cell culture system s have made them an am e­ nable tool for studying many aspects of biocide research; screening, m etabolism , m ode of action and resistance. Cell cultures are "m ulti-hom ogeneous" and each hom ogeneous state may be analog­ ous to different plant parts. Cultures can be hom ogeneously green, hom ogeneously w hite in either a hom ogeneously exponential or stationary phase of growth. Exam ples are be presented showing why no sin gle hom ogeneous state should be envisaged to be analogous to a whole plant in biocide studies. Because of the possibility of more uniform herbicide application, culture systems have a use in isolatin g resistant crop strains, at higher selection pressures and much higher "p lan t" densities than is possib le in the field, with recent successes. Protoplast fusion techniques may allow the transfer of gen etic resistance between related but genetically incom patible crop species. 
  Reference    Z. Naturforsch. 34c, 905—913 (1979); received M ay 24 1979 
  Published    1979 
  Keywords    Callus Cultures, Suspension Cultures, H erbicide Action, H erbicide M etabolism, H erbicide R esistance 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0905.pdf 
 Identifier    ZNC-1979-34c-0905 
 Volume    34