| | 2 | Author
| Joachim Hilfenhaus, HermannE. Karges | Requires cookie* | | | Title
| Growth Inhibition of Human Lymphoblastoid Cells by Interferon Preparations, Obtained from Human Leukocytes  | | | | Abstract
| The growth inhibition of human lymphoblastoid cells by human interferon preparations (HIF) and a mock-interferon preparation (Mock-IF) was studied. HIF was induced in suspensions of primary human leukocytes by Newcastle Disease Virus. Mock-IF was prepared from uninduced cell suspension of a comparable batch. The highest degree of inhibition of cell propagation was ob tained on the EBV producer line P3HR1, the inhibition of the cell lines Raji and SKL 1 was significantly lower, no effect was observed on the cell line RPMI 1788 originating from a healthy donor. Mock-IF also caused a growth inhibition but to a lower degree as HIF. HIF and Mock-IF are similar in the following properties: 1. Stability at pH 2 at 4 °C for four days, 2. sensitivity to tryptic digestion, and 3. molecular weights in the range between 5,000 and 40,000 daltons. | | | |
Reference
| (Z. Naturforsch. 29c, 618 [1974]; received May 13/July 11 1974) | | | |
Published
| 1974 | | | |
Keywords
| Interferon, Mock-Interferon, Growth Inhibition, Human Lymphoblastoid Cells, Epstein Barr Virus | | | |
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| | | | | TEI-XML for
| default:Reihe_C/29/ZNC-1974-29c-0618.pdf | | | | Identifier
| ZNC-1974-29c-0618 | | | | Volume
| 29 | |
| 4 | Author
| M. Wink | Requires cookie* | | | Title
| Chemical Defense of Leguminosae. Are Quinolizidine Alkaloids Part of the Antimicrobial Defense System of Lupins? | | | | Abstract
| Growth o f 6 bacteria (Serratia marcescens, Bacillus megaterium , Bacillus subtilis, Streptococcus viridis, Micrococcus luteus, and M ycobacterium ph lei) was inhibited by 50% if the growth m edium contained sparteine at concentrations between 0 .5 -1 0 m M . Total growth inhibition, which was bacteriostatic in nature, was achieved at 20 m M . The growth o f 6 phytopathogenic fungi was also affected: at a sparteine concentration o f 15 m M the growth o f Alternaria porri was reduced by 40% as compared to the untreated control. R espective values were 18% inhibition for Piricularia oryzae, 33% for H elminthosporium carbonum, 15% for R hizoctonia solani, 5% for Fusarium oxysporum, and 42% for Asperquillus oryzae. Since the concentrations o f quinolizidine alkaloids range from 1 -2 0 0 m M (roots, leaves, or stems) or 1 0 -2 0 0 m m ol/k g (seeds) in Legum inosae, it is discussed whether quinolizidine alkaloids are involved in the antim icrobial defense o f lupins, in addition to their potential role as allelopathic or herbivore repellent defense com pounds. | | | |
Reference
| Z. Naturforsch. 39c, 548 (1984); received January 23 1984 | | | |
Published
| 1984 | | | |
Keywords
| Quinolizidine Alkaloids, Growth Inhibition, Bacteria, Fungi, Antim icrobial Defense | | | |
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| default:Reihe_C/39/ZNC-1984-39c-0548.pdf | | | | Identifier
| ZNC-1984-39c-0548 | | | | Volume
| 39 | |
| 5 | Author
| W. Erner, K. Roll, StefanP. Ostius, F. Riedhelm, S. Chneider | Requires cookie* | | | Title
| Proliferation Kinetics and Metabolic Features of in vitro Grown Ehrlich Ascites Tumor Cells in the Presence of Exogenous Pyruvate | | | | Abstract
| The proliferation o f in vitro grown Ehrlich ascites tum or cells is inhibited by pyruvate concen trations > 2 m M . In the presence o f 4 —5 m M pyruvate the growth is reduced to about 50% , in the presence o f 20 mM to about 5 — 10%. Viability o f the cells is not severely affected. Increase o f D N A corresponds to the cell growth. On recultivation in pyruvate free standard m edium , growth is nearly normal. Flow cytometric analyses o f the proliferation kinetics of the cells in the presence o f 20 m M pyruvate revealed a retardation o f the passage o f all phases o f the cell cycle. N o phase specific effects could be detected though the S-and G 2M -p hase are more afflicted than G l . The growth inhibition o f E A T cells by pyruvate seem s to depend on the presence of glucose. Exogenous pyruvate (> 1 —2 m M) causes an activation o f pyruvate dehydrogenase, a reduction o f lactate production from glucose and a stimulation o f lipid biosynthesis; the N A D /N A D H ratio of the cells is reduced and a rise o f glycolytic interm ediates beyond glyceraldehyde-3-phosphate dehydrogenase is observed. Maximal activation of pyruvate dehydrogenase by non toxic concen trations o f dichloroacetate is also accom pagnied by an inhibition o f cell growth. It is suggested that an increase o f glyceraldehyde-3-phosphate level and the changes in the redox state o f the cells are o f relevance for the inhibition o f cell growth by pyruvate. 100—500 ^im exogenous glycer-aldehyde-3-phosphate strongly inhibited cell growth. | | | |
Reference
| Z. Naturforsch. 41c, 787—794 (1986); received March 20 1986 | | | |
Published
| 1986 | | | |
Keywords
| Ehrlich A scites Tumor Cells, Growth Inhibition, Pyruvate, M etabolism | | | |
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| default:Reihe_C/41/ZNC-1986-41c-0787.pdf | | | | Identifier
| ZNC-1986-41c-0787 | | | | Volume
| 41 | |
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