| | 1 | Author
| W. Erner, K. Roll, StefanP. Ostius, F. Riedhelm, S. Chneider | Requires cookie* | | | Title
| Proliferation Kinetics and Metabolic Features of in vitro Grown Ehrlich Ascites Tumor Cells in the Presence of Exogenous Pyruvate  | | | | Abstract
| The proliferation o f in vitro grown Ehrlich ascites tum or cells is inhibited by pyruvate concen trations > 2 m M . In the presence o f 4 —5 m M pyruvate the growth is reduced to about 50% , in the presence o f 20 mM to about 5 — 10%. Viability o f the cells is not severely affected. Increase o f D N A corresponds to the cell growth. On recultivation in pyruvate free standard m edium , growth is nearly normal. Flow cytometric analyses o f the proliferation kinetics of the cells in the presence o f 20 m M pyruvate revealed a retardation o f the passage o f all phases o f the cell cycle. N o phase specific effects could be detected though the S-and G 2M -p hase are more afflicted than G l . The growth inhibition o f E A T cells by pyruvate seem s to depend on the presence of glucose. Exogenous pyruvate (> 1 —2 m M) causes an activation o f pyruvate dehydrogenase, a reduction o f lactate production from glucose and a stimulation o f lipid biosynthesis; the N A D /N A D H ratio of the cells is reduced and a rise o f glycolytic interm ediates beyond glyceraldehyde-3-phosphate dehydrogenase is observed. Maximal activation of pyruvate dehydrogenase by non toxic concen trations o f dichloroacetate is also accom pagnied by an inhibition o f cell growth. It is suggested that an increase o f glyceraldehyde-3-phosphate level and the changes in the redox state o f the cells are o f relevance for the inhibition o f cell growth by pyruvate. 100—500 ^im exogenous glycer-aldehyde-3-phosphate strongly inhibited cell growth. | | | |
Reference
| Z. Naturforsch. 41c, 787—794 (1986); received March 20 1986 | | | |
Published
| 1986 | | | |
Keywords
| Ehrlich A scites Tumor Cells, Growth Inhibition, Pyruvate, M etabolism | | | |
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| ZNC-1986-41c-0787 | | | | Volume
| 41 | |
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