| | 1 | Author
| K. Stich3, H. Halbwirth3, F. Wurst3, G. Forkmannb | Requires cookie* | | | Title
| UDP-GIucose: Flavonol 7-O-glucosyItransferase Activity in Flower Extracts o f Chrysanthemum segetum  | | | | Abstract
| The yellow colour of Chrysanthemum segetum petals is due to the presence o f the 7-0-glucosides of quercetin and particularly gossypetin (8-hydroxyquercetin). In petal extracts of C. segetum an enzyme was demonstrated which catalyzes the transfer o f the glucosyl moiety o f uridine 5'-diphosphoglucose (U D P G) to the 7-hydroxyl group of flavonols with gossypetin and quercetin as the best substrates. Besides flavonols flavanones and flavones were found to be glucosylated in the 7-position. The pH-optimum of the reaction highly depended on the substrate used. With quercetin as substrate, maximal enzyme activity occurred at a pH o f 8.25 and a temperature of 25 °C, but 7-O-glucosylation also proceeded at low temper atures. Studies on temperature stability revealed, that there was no influence on the glucosy-lation reaction up to 40 °C. Higher temperatures led to a loss of enzyme activity. Using gossypetin as a substrate a similar course of temperature stability was observed. Addition of M g2+, Ca2+ and K C N slightly stimulated 7-O-glucosylation, whereas C o2+, Cu2+, Fe2+, Hg2+, p-hydroxymercuribenzoate and N-ethylmaleimide showed a strong inhibitory effect. Addi tional enzymatic studies were performed with the commercial strain " Stern des Orients" where gossypetin 7-O-glucoside is restricted to the inner parts of the petals. For enzyme extracts from both parts of the petals gossypetin was found to be the most attractive sub strate. In comparison to quercetin (133.4 [.ikat / kg protein) an about three times higher specific activity of the 7-0-glucosyltransferase(s) was determined with gossypetin (382.1 (.ikat/ kg protein) as substrate, indicating that hydroxylation o f quercetin in 8-position to gossypetin precedes 7-O-glucosylation. | | | |
Reference
| Z. Naturforsch. 52c, 153—158 (1997); received November 11/ | | | |
Published
| 1997 | | | |
Keywords
| Chrysanthemum segetum, UDP-Glucose: Flavonol 7-O-glucosyltransferase, Quercetin, Gossypetin, Flavonoids | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/52/ZNC-1997-52c-0153.pdf | | | | Identifier
| ZNC-1997-52c-0153 | | | | Volume
| 52 | |
|
