Go toArchive
Browse byFacets
Bookbag ( 0 )
'Glycoconjugate' in keywords Facet   Publication Year 1997  [X]
Results  1 Item
Sorted by   
Publication Year
1Author    StefanL. Atza, RalfG B ErgerRequires cookie*
 Title    l-0-fra#is-Cinnamoyl-/?-D-glucopyranose: Alcohol Cinnamoyltransferase Activity in Fruits of Cape Gooseberry (Physalis peruviana L.)  
 Abstract    Methyl and ethyl cinnamate are aroma volatiles frequently occurring in fruits. Evidence was obtained that the enzymatic transfer of cinnamic acid to endogenous alcohols present in fruits (methanol, ethanol, 1-propanol) depended on energy-rich 1-O-glycosyl esters of cin­ namic acid which served as acyl donor molecules. A putative 1-O-fra/w-cinnamoyl-^-D-gluco-pyranose; alcohol cinnamoyltransferase from cape gooseberry (Physalis peruviana L.) was active towards l-0-rra«s-cinnamoyl-/3-D-glucopyranose and l-0-rrans-cinnamoyl-/3-D-gentio-biose. The enzyme was purified 290-fold by a protocol including ammonium sulphate precipi­ tation, solubilization by Triton X-100, gel permeation and affinity chromatography on conca-navalin A. The acidic glycoprotein {p i = 4.8) most probably is membrane bound. The distribution of alcohol cinnamoyltransferase activity in gel chromatography fractions suggests a native Mr of 75,000. For l-O-mms-cinnamoyl-ß-D-glucopyranose, an apparent K m of 69 ^im was determined. At pH > 6.0, non-enzymatic transesterification superposes the enzymatic transformation. 
  Reference    Z. Naturforsch. 52c, 747—755 (1997); received June 12/July 28 1997 
  Published    1997 
  Keywords    Phenylpropanoid Metabolism, Glycoconjugate, Flavour Precursor, Aroma Ester, Methyl Cinnamate 
  Similar Items    Find
 TEI-XML for    default:Reihe_C/52/ZNC-1997-52c-0747.pdf 
 Identifier    ZNC-1997-52c-0747 
 Volume    52