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'Glyceollin' in keywords
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1985 (1)
1982 (1)
1Author    P. Moesta, U. Seydel, B. Lindner, H. G. RisebachRequires cookie*
 Title    Detection of Glyceollin on the Cellular Level in Infected Soybean by Laser Microprobe Mass Analysis  
 Abstract    Laser microprobe mass analysis was explored as a m ethod for the detection at the cellular level of the phytoalexin glyceollin in soybean cotyledons infected with an incom patible race of Phy­ tophthora megasperma f. sp. glycinea. For LAMMA® analysis 10 |im freeze microtome sections which were freeze-dried on copper grids were used. The LAM M A spectrum of glyceollin (isomer I) shows a characteristic peak at m /e = 321 which can be attributed to M -O H +. This peak was also present in the spectra of infected regions from a cotyledon b u t was completely absent in the spec­ tra of uninfected tissue. One hundred and fifty LAM M A spectra were taken along a line per­ pendicular to the border line o f infection. A steep rise in glyceollin content toward the infected area was observed. This is the First tim e th at such highly localized glyceollin accumulation has been shown at the cellular level. The results show th at LAM M A analysis is suitable for the de­ tection of organic molecules in biological tissues w ith high lateral resolution. 
  Reference    Z. Naturforsch. 37c, 748—751 (1982); received May 19 1982 
  Published    1982 
  Keywords    Glyceollin, Phytoalexins, Soybean, Glycine m ax, L, LAM MA 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0748.pdf 
 Identifier    ZNC-1982-37c-0748 
 Volume    37 
2Author    DietrichW. Erner, RobertB M Ellor, MichaelG H Ahn, Hans GrisebachRequires cookie*
 Title    Soybean Root Response to Symbiotic Infection Glyceollin I Accumulation in an Ineffective Type of Soybean Nodules with an Early Loss of the Peribacteroid Membrane  
 Abstract    A glyceollin I accumulation o f about 6000 pm ol • mg dry weight-, a tenfold increase above control root tissue, was found in one type o f nodule from Glycine m ax which had been infected with a fix ~ strain (61-A-24) o f Rhizobium japonicum . In nodules infected with one other ineffective (ßx~) strain o f Rhizobium japonicum (RH 31-Marburg) or with two f i x + strains o f Rhizobium japonicum (61 -A -101 and U SD A 110) no increase in glyceollin I concentrations above control values was found at either 20 d or 34 d after infection. N odules infected with Rhizobium japonicum 61-A-24 are distinguished by an early loss o f the peribacteroid membrane in the infected host cell, whilst the bacteroids them selves remain stable. 
  Reference    Z. Naturforsch. 40c, 179—181 (1985); received N ovem ber 23 1984 
  Published    1985 
  Keywords    Glyceollin, Glycine max, Nitrogen Fixation, N odules, Peribacteroid Membrane 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0179.pdf 
 Identifier    ZNC-1985-40c-0179 
 Volume    40