ZfN: Search Results

1	Author	DaivaA. Bironaitė, N. Arim, K. Čėnas, JuozasJ. Kulys, AlexanderG. Medentsev, VasiliyK. Akimenko, P. A. Karplus, E. F. Pai, G. E. Schulz, EurJ. Biochem	Requires cookie*
	Title
	Abstract	Fully substituted quinones including som e naturally occurring oxyquinones acted as inhibitors o f yeast gluta thione reductase (EC 1.6.4.2). They were competitive, mixed or uncompetitive inhibitors for N A D P H , possess ing K j in the range o f 1 -2 0 0 \|iM and uncompetitive in hibitors for glutathione. Rhein (4,5-dioxy-9,10-anthra-quinone-2-carbonic acid) and 9,10-phenanthrenequi-none were the most effective inhibitors. It is concluded that certain quinones can bind to the N A D P(H)-binding site and to the heteroaromatics binding site at the inter face domain (o f the enzyme.
	Reference	Z. Naturforsch. 46c, 966—9 (1991); received March 4/M ay 71991. 178 6 9 3 -7 0 3 1989
	Published	1991
	Keywords	Glutathione Reductase, Inhibition, Quinones
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	TEI-XML for	default:Reihe_C/46/ZNC-1991-46c-0966_n.pdf
	Identifier	ZNC-1991-46c-0966_n
	Volume	46

2	Author	AnnaM M Ata, Juan López-Barea	Requires cookie*
	Title	Purification by Affinity Chromatography of Glutathione Reductase (EC 1.6.4.2) from Escherichia coli and Characterization of such Enzyme
	Abstract	The glutathione reductase from Escherichia coli strain S33 was purified to homogeneity by a simple and fast procedure consisting of two affinity chromatography steps. After 40-80% ammonium sulfate fractionation, the enzyme was adsorbed to an N 6-2'.5'-ADP-Sepharose affinity column from which it was specifically eluted by a 0 -10 m M N ADP+ linear gradient. The enzyme was finally purified to homogeneity after a second affinity chromatography step in a C8-ATPR-Sepharose column, from which it was eluted by means of the same N AD P+ gradient.
	Reference	Z. Naturforsch. 39c, 908—915 (1984); received December 16 1983/April 26 1984
	Published	1984
	Keywords	Escherichia coli, Glutathione Reductase, Purification by Affinity Chromatography, Molecular and Kinetic Characterization, Redox Inactivation under Reducing Conditions
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	TEI-XML for	default:Reihe_C/39/ZNC-1984-39c-0908.pdf
	Identifier	ZNC-1984-39c-0908
	Volume	39