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1984 (1)
1980 (2)
1Author    Dieter StrackRequires cookie*
 Title    Enzymatic Synthesis of 1-Sinapoylglucose from Free Sinapic Acid and UDP-Glucose by a Cell-Free System from Raphanus sativus Seedlings  
 Abstract    Protein extracts from seedlings of Raphanus sativus catalyze the transfer of the glucosyl moiety of UDP-glucose to the carboxyl group o f phenolic acids. Enzymatic activity was determined spec-trophotometrically by measuring the increase in absorbance at 360 nm and/or by the aid of high performance liquid chromatography (HPLC). From 12 phenolic acids tested as acceptors, sinapic acid was by far the best substrate. The glu-cosyltransfer to sinapic acid has a pH optimum near 7 and requires as SH group for activity, p-Chloromercuribenzoate (PCMB) inhibits activity, which can be restored by the addition of di-thiothreitol (DTT). The formation o f 1-sinapoylglucose was found to be a reversible reaction, sin­ ce the addition of UDP results in a breakdown o f the ester. 
  Reference    Z. Naturforsch. 35c, 204—2 (1980); received December 28 1979 
  Published    1980 
  Keywords    Raphanus, Brassicaceae, Phenylpropanoid Metabolism, Glucose Ester, Esterification 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0204.pdf 
 Identifier    ZNC-1980-35c-0204 
 Volume    35 
2Author    D. Ieter, Strack An, M. Aria Bokern, Jochen Berlin An D, Sabine SiegRequires cookie*
 Title    Metabolie Activity of Hydroxycinnamic Acid Glucose Esters in Cell Suspension Cultures of Chenopodium rubrum  
 Abstract    Cell suspension cultures o f Chenopodium rubrum accumulate high amounts of metabolically active glucose esters of ^?-coumaric and ferulic acid. Pulse-labelling experiments using [14C]phe-nylalanine and application o f the phenylalanine ammonia-lyase (PAL) inhibitor L-a-aminooxy-/3-pnenylpropionic acid (AOPP) revealed that the glucose esters are subject to high turnover. A considerable portion o f the ferulic acid was found as an insoluble component, probably bound to cell wall material. Application of m-fluoro-DL-tyrosine (MFT), an effective inhibitor o f tyrosine biosynthesis via L-arogenate, markedly increased the amount of 1-p-coumaroyl-and 1-feruloyl-glucose accumulated. 
  Reference    Z. Naturforsch. 39c, 902—907 (1984); received May 16 1984 
  Published    1984 
  Keywords    Chenopodium rubrum, Cell Culture, Hydroxycinnamic Acid, Glucose Ester, Betalain 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0902.pdf 
 Identifier    ZNC-1984-39c-0902 
 Volume    39 
3Author    A. Fleuriet, J. J. Macheix, R. Suen, R. K. IbrahimRequires cookie*
 Title    Partial Purification and Some Properties of a Hydroxycinnamoyl Glucosyltransferase from Tomato Fruits  
 Abstract    A glucosyltransferase was isolated from im mature "cherry" tom atoes and was partially purified (200-fold) by am m onium sulphate precipitation and successive chrom atography on Sephadex G-100 and DEAE-cellulose columns. The enzyme utilised the free hydroxycinnamic acids and UDP-glucose in the form ation o f their respective glucosides (pH 8.0) and glucose esters (pH 7.0); but did not accept the CoA thiolesters o f HCAs in the presence of glucose-1-phosphate. The constant glucoside/glucose ester ratio observed during purification suggests that both reactions are catalysed by the sam e enzyme. The K m values for /»-coumaric, caffeic, ferulic and sinapic acids were 0.8, 1.5, 1.4 and 2.5 hm, respectively. W ith ferulic acid as substrate, the K m value for U D PG was 10 hm. The enzyme required an -S H group for activity and the reaction was strongly inhibited by EDTA, divalent metal ions and UDP. 
  Reference    Z. Naturforsch. 35c, 967—9 (1980); received August 14 1980 
  Published    1980 
  Keywords    Glucosyltransferase, Glucosides, Glucose Esters, Hydroxycinnamic Acids, Enzyme Purification and Properties, Tom ato Fruit, Lycopersicum esculentum (Solanaceae) 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0967.pdf 
 Identifier    ZNC-1980-35c-0967 
 Volume    35