ZfN: Search Results

1	Author	Dieter Strack	Requires cookie*
	Title	Enzymatic Synthesis of 1-Sinapoylglucose from Free Sinapic Acid and UDP-Glucose by a Cell-Free System from Raphanus sativus Seedlings
	Abstract	Protein extracts from seedlings of Raphanus sativus catalyze the transfer of the glucosyl moiety of UDP-glucose to the carboxyl group o f phenolic acids. Enzymatic activity was determined spec-trophotometrically by measuring the increase in absorbance at 360 nm and/or by the aid of high performance liquid chromatography (HPLC). From 12 phenolic acids tested as acceptors, sinapic acid was by far the best substrate. The glu-cosyltransfer to sinapic acid has a pH optimum near 7 and requires as SH group for activity, p-Chloromercuribenzoate (PCMB) inhibits activity, which can be restored by the addition of di-thiothreitol (DTT). The formation o f 1-sinapoylglucose was found to be a reversible reaction, sin ce the addition of UDP results in a breakdown o f the ester.
	Reference	Z. Naturforsch. 35c, 204—2 (1980); received December 28 1979
	Published	1980
	Keywords	Raphanus, Brassicaceae, Phenylpropanoid Metabolism, Glucose Ester, Esterification
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0204.pdf
	Identifier	ZNC-1980-35c-0204
	Volume	35

2	Author	D. Ieter, Strack An, M. Aria Bokern, Jochen Berlin An D, Sabine Sieg	Requires cookie*
	Title	Metabolie Activity of Hydroxycinnamic Acid Glucose Esters in Cell Suspension Cultures of Chenopodium rubrum
	Abstract	Cell suspension cultures o f Chenopodium rubrum accumulate high amounts of metabolically active glucose esters of ^?-coumaric and ferulic acid. Pulse-labelling experiments using [14C]phe-nylalanine and application o f the phenylalanine ammonia-lyase (PAL) inhibitor L-a-aminooxy-/3-pnenylpropionic acid (AOPP) revealed that the glucose esters are subject to high turnover. A considerable portion o f the ferulic acid was found as an insoluble component, probably bound to cell wall material. Application of m-fluoro-DL-tyrosine (MFT), an effective inhibitor o f tyrosine biosynthesis via L-arogenate, markedly increased the amount of 1-p-coumaroyl-and 1-feruloyl-glucose accumulated.
	Reference	Z. Naturforsch. 39c, 902—907 (1984); received May 16 1984
	Published	1984
	Keywords	Chenopodium rubrum, Cell Culture, Hydroxycinnamic Acid, Glucose Ester, Betalain
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	TEI-XML for	default:Reihe_C/39/ZNC-1984-39c-0902.pdf
	Identifier	ZNC-1984-39c-0902
	Volume	39

3	Author	A. Fleuriet, J. J. Macheix, R. Suen, R. K. Ibrahim	Requires cookie*
	Title	Partial Purification and Some Properties of a Hydroxycinnamoyl Glucosyltransferase from Tomato Fruits
	Abstract	A glucosyltransferase was isolated from im mature "cherry" tom atoes and was partially purified (200-fold) by am m onium sulphate precipitation and successive chrom atography on Sephadex G-100 and DEAE-cellulose columns. The enzyme utilised the free hydroxycinnamic acids and UDP-glucose in the form ation o f their respective glucosides (pH 8.0) and glucose esters (pH 7.0); but did not accept the CoA thiolesters o f HCAs in the presence of glucose-1-phosphate. The constant glucoside/glucose ester ratio observed during purification suggests that both reactions are catalysed by the sam e enzyme. The K m values for /»-coumaric, caffeic, ferulic and sinapic acids were 0.8, 1.5, 1.4 and 2.5 hm, respectively. W ith ferulic acid as substrate, the K m value for U D PG was 10 hm. The enzyme required an -S H group for activity and the reaction was strongly inhibited by EDTA, divalent metal ions and UDP.
	Reference	Z. Naturforsch. 35c, 967—9 (1980); received August 14 1980
	Published	1980
	Keywords	Glucosyltransferase, Glucosides, Glucose Esters, Hydroxycinnamic Acids, Enzyme Purification and Properties, Tom ato Fruit, Lycopersicum esculentum (Solanaceae)
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-0967.pdf
	Identifier	ZNC-1980-35c-0967
	Volume	35