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'GTP Binding Protein' in keywords Facet   Publication Year 1985  [X]
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1985[X]
1Author    A. Ndreas Schleicher, KlausPeter HofmannRequires cookie*
 Title    Proton Uptake by Light Induced Interaction between Rhodopsin and G-Protein  
 Abstract    The light-induced proton uptake of rod outer segment disc membranes has been investigated in the absence and presence of G-protein. Proton uptake was measured as the alkalisation of the suspending medium using a pH electrode and/or the indicator dye bromocresol purple. It was found that besides the known proton uptake of photolysed rhodopsin additional uptake of one proton accompanies formation of the complex between rhodopsin and G-protein. No measurable proton uptake was found under conditions of rapid redissociation of the complex indicating an only transient protonation during its lifetime. Proton uptake was the same in washed membra­ nes recombined with G-protein and in ordinarily stacked rod outer segments. The additional proton uptake reported here is not due to enhanced metarhodopsin II. 
  Reference    Z. Naturforsch. 40c, 400—405 (1985); received January 14/February 25 1985 
  Published    1985 
  Keywords    Photoreceptor, Protein-Protein Interaction, GTP-Binding Protein, Rhodopsin 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0400.pdf 
 Identifier    ZNC-1985-40c-0400 
 Volume    40