| | 1 | Author
| Petra Sperling, Ute Hammer, Wolfgang Friedt, Ernst Heinz | Requires cookie* | | | Title
| High Oleic Sunflower: Studies on Composition and Desaturation of Acyl Groups in Different Lipids and Organs  | | | | Abstract
| A selection o f lipids from achenes, cotyledons after germination, roots and leaves o f normal and high oleic varieties o f sunflower were analyzed with regard to their fatty acid profiles. The lipids included triacylglycerol and phosphatidylcholine as ER-made components and mono-and digalactosyl diacylglycerol as plastid-localized glycolipids. A comparison o f fatty acid pat terns showed that the block in oleate desaturation o f the high oleic variety is confined to the ER o f fat accumulating embryos, but that upon germination the oleate desaturation in the cotyledonary ER is rapidly derepressed. These data are supported by enzymatic experiments. In m icrosomes from maturing fruits o f the high oleic variety oleoyl-phosphatidylcholine de-saturase could not be detected, whereas o leo y l-C o A : lyso-phosphatidylcholine acyltransferase and com ponents o f the microsomal electron transport chains were not affected. A correlation in the expression o f desaturation blocks in seed and root fatty acids as observed in mutants o f other species was not observed which, therefore, cannot be generalized. Our data are discussed in terms o f the existence o f two ER-specific oleate desaturase activities. | | | |
Reference
| Z. Naturforsch. 45c, 166 (1990); received November 20 1989 | | | |
Published
| 1990 | | | |
Keywords
| Triacylglycerols, G alactolipids, Phosphatidylcholine, Cotyledons, Leaves, Roots | | | |
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| default:Reihe_C/45/ZNC-1990-45c-0166.pdf | | | | Identifier
| ZNC-1990-45c-0166 | | | | Volume
| 45 | |
| 2 | Author
| Britta Verhoek, RenateKäthe Haas, Michael Wrage, Linscheid, Ernst Heinz | Requires cookie* | | | Title
| Lipids and Enzymatic Activities in Vacuolar Membranes Isolated via Protoplasts from Oat Primary Leaves | | | | Abstract
| Vacuoles were released from oat (Avena sativa) mesophyll protoplasts and purified by sed i mentation and flotation. Disruption o f isolated vacuoles follow ed by density gradient centri fugation gave two m embrane bands which after com bination were further purified on sucrose gradients. A significant contam ination by microbodies, thylakoids, m itochondria, endoplasm ic reticulum and Golgi membranes can be excluded, whereas markers for plasma m em brane and chloroplast envelope were present in the final membrane preparation. In the purified membrane fraction the follow ing enzym atic activities were detected: N A D H -cytochrome C reductase E.C. 1.6.2.2, ATPase E.C. 3.6.1.3, UD PG : sterol glucosyltransferase, UDP-Gal: diacylglycerol galactosyltransferase E.C. 2.4.1.46, glucan synthetase II, C D P-choline: diacylglycerol phosphocholinetransferase E.C. 2.7.8.2, formation o f acylgalactosyl diacylglycerol and acyl-CoA thioesterase E.C. 3.1.2.2. N on e o f these can be considered to be sp ecific for the tonoplast. Acid phosphatase E.C. 3.1.3.2 was present in the cell sap. The vacuolar membranes contain phospholipids and glycolipids o f the most com plex com posi tion found so far in a membrane system isolated from m esophyll protoplasts. About h alf o f the glycolipids were accounted for by glycosyl diacylglycerols usually considered to be confined to plastids. Steryl glycosides and acyl steryl glycosides were other prominent glycolipids. A cere-broside was the predominating lipid com ponent o f this membrane preparation. | | | |
Reference
| Z. Naturforsch. 38c, 770—7 (1983); received May 5 1983 | | | |
Published
| 1983 | | | |
Keywords
| Contamination, Microsomal Enzymes, G alactolipids, Steryl G lycosides, Cerebrosides | | | |
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| default:Reihe_C/38/ZNC-1983-38c-0770.pdf | | | | Identifier
| ZNC-1983-38c-0770 | | | | Volume
| 38 | |
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