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1982 (1)
1980 (1)
1Author    Peter Scheurich, Ulrich Zimmermann, Maja Mischel, Ingolf LamprechtRequires cookie*
 Title    Membrane Fusion and Deformation of Red Blood Cells by Electric Fields  
 Abstract    Human red blood cells suspended in a slightly hypotonic solution o f low electric conductivity were exposed to an inhomogeneous and alternating electric field (sine wave, 30 V peak-to-peak value, electrode distance 120 |im, 0.5 to 2 MHz). Due to the dielectrophoretic effect the cells align parallel to the field lines under the formation o f pearl chains. At high voltages (10 V amplitude) membrane fusion is observed between the adhered red blood cells in the pearl chains, whereby the chains become attached to the electrodes. In contrast to the pearl chains observed at voltages o f up to 5 V amplitude the resulting fused and uniform aggregates which exhibit no recognisable individual cells under the light microscope, remain stable, even after the alternating electric field has been switched off or after haemolysis in response to osmotic shock. The fused aggregates are highly elastic. If the field strength o f the applied alternating electric field is further increased they are stretched in the direction o f the opposite electrode. Frequently, bridges are formed between the two electrodes. The uniform bridges remain stable for some time even in the absence o f an electric field. The possibility o f cell fusion and its initiation by electrical breakdown of the cell membranes are discussed. 
  Reference    Z. Naturforsch. 35c, 1081—1085 (1980); received August 21 1980 
  Published    1980 
  Keywords    Deformability, Dielectrophoresis, Electric Breakdown, Erythrocytes, Fusion 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-1081.pdf 
 Identifier    ZNC-1980-35c-1081 
 Volume    35 
2Author    Fumio Yoshii, Isao KaetsuRequires cookie*
 Title    Effect of Water Soluble Polymer, Polyethyleneglycol, and Glass-Forming Compounds on Cell Fusion  
 Abstract    The cell fusion of Molt T-cells was investigated in the presence of water soluble polymer, polyethyleneglycol (PEG) and glass-forming monomers. In cell fusion with PEG only, inactivation of the cell occurred within 2 min. However, in the presence of PEG and water soluble polymer, most of the fused and unfused cells lived even after fusion times of more than 10 min. It was observed that water soluble polymer prevented the inactivation of cells during fusion as a protectant. As the result, ratio of fused cells increased in the presence of water soluble polymers. Some glass-forming monomers used as new fusogens such as M-23G (« = 23) and M-50G (/? = 50) monomers having long oxyethylene chains in the methoxypolyethyleneglycol methacry­ late, C H 30 -(-C H 2C H 2-0)" —C O -C (C H j) = CH 2 as well as PEG #2000 indicated large promoting and protecting effects on cell fusion. 
  Reference    Z. Naturforsch. 37c, 1234—1239 (1982); received March 1/August 10 1982 
  Published    1982 
  Keywords    Water Soluble Polymer, Molt T-Cell, Fusion, Monomer, Fusogen, Polyethyleneglycol 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-1234.pdf 
 Identifier    ZNC-1982-37c-1234 
 Volume    37