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1982 (1)
1Author    Charles Tanford, DwightW. MartinRequires cookie*
 Title    Equilibrium Constants for Some Steps of the Reaction Cycle of the Sarcoplasmic Reticulum Calcium Pump  
 Abstract    This paper summarizes true equilibrium m easurements for som e partial reactions o f the sar­ coplasmic reticulum calcium pum p transport cycle. The most important result is the estim ation o f the equilibrium constant for the interconversion o f the two major conformational states o f the protein, E (Ca2+ binding sites facing the cytoplasm) and E' (C a2+ binding sides facing the sar­ coplasm ic reticulum lumen). The value o f K0 = [E']/[E] cannot be evaluated directly by any method available at present, but observed cooperativity in the binding o f Mg2+ and Ca2+ to un-liganded protein strongly indictes that K0 ^ 1-The m ost probable value, valid within an order o f magnitude, is K0 ~ 103, i.e., the E' state is more stable than the E state by about 4 kcal/m ol. 
  Reference    Z. Naturforsch. 37c, 522 (1982); received February 19 1982 
  Published    1982 
  Keywords    Ca2+ ATPase, Free Energy Transduction, Active Transport 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0522.pdf 
 Identifier    ZNC-1982-37c-0522 
 Volume    37