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'Formycin' in keywords Facet   Publication Year 1983  [X]
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1Author    Jacek Wierzchowski, David ShugarRequires cookie*
 Title    Sensitive Fluorimetric Assay for Adenosine Deaminase with Formycin as Substrate; and Substrate and Inhibitor Properties of some Pyrazolopyrimidine and Related Analogues  
 Abstract    The nucleoside antibiotic formycin, 7-amino-3-(/?-D-ribofuranosyl)pyrazolo(4,3-d)pyrimidine, a structural analogue of adenosine, is deaminated about 10-fold faster by adenosine deaminase than adenosine itself, and is therefore a superior substrate for both routine assays and kinetic studies with the purified enzyme. The luminescence properties o f formycin have been profited from to develop a fluorimetric assay for adenosine deaminase which is considerably more sensi­ tive than the spectrophotometric procedure widely employed with adenosine as substrate. Exam­ ples are presented of its application to routine assays of adenosine deaminase levels in cellular extracts, as well as to kinetic studies with the purified enzyme, including the properties o f some pyrazolopyrimidine and purine substrates and inhibitors. 
  Reference    Z. Naturforsch. 38c, 67—7 (1983); received August 23 1982 
  Published    1983 
  Keywords    Formycin, Pyrazolopyrimidines, Adenosine Deaminase, Fluorimetric Assay, Inhibitors 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0067.pdf 
 Identifier    ZNC-1983-38c-0067 
 Volume    38